SYNOPSISCharacteristics of the goose hepatitis virus strain SHM 319 were studied in goose embryos and tissue cultures of avian origin. The virus was found to be resistant to both ether and sodium deoxycholate (0.25%). The growth of the virus was inhibited by 5-iodo-2-deoxyuridine. Virus infectivity was not affected by heating at 56°C for 3 hours, exposure to pH 3.0, formalin (1:1000), and other inactivating chemicals. Cell culture systems of three avian species were inoculated with GVH-SHM 319. Extensive cytopathic effect was produced only in goose cell cultures. No virus replication was observed in chicken or White Pekin duck cells. Fluorescent antibody staining revealed granular nuclear staining in infected susceptible tissue cultures. MayGrünwald-Giemsa-staining of infected tissue culture cells showed formation of mainly intranuclear inclusion bodies. Millipore filtration procedures revealed a particle size less than 50 nm. Hemagglutination was not observed. Precipitating antibodies could not be detected in GHV hyperimmunized birds. Neutralization tests could not demonstrate a relationship to known viruses of waterfowl, including duck virus enteritis and duck virus hepatitis. A certain discrepancy in the neutralization test with Hungarian goose sera is discussed.

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Zusammenfassung Aus einer Jungentenherde, die plötzlich mit einer hohen Mortalitätsrate erkrankte (20%), wurde ein hämagglutinierendes RNA‐Virus isoliert, das ein gemeinsames gruppenspezifisches Antigen mit Influenza‐A‐Viren besitzt und serologisch der Gruppe 6 der aviären Influenzaviren zuzuordnen ist. Es besitzt die bekannten biochemischen und biophysikalischen Eigenschaften der Influenzaviren. Bei experimenteller Infektion erwies sich der isolierte Stamm als nicht sehr pathogen. Das Virus konnte von den Conjunktiven experimentell infizierter Tiere reisoliert werden. Summary Isolation, characterisation and biological properties of a strain of influenza A virus of Group 6 isolated from ducks (Duck/Germany 1868/68) From a group of young ducks which suddenly suffered a high mortality rate (20 %), a haemagglutinating RNA virus was isolated which possessed a common group‐specific antigen with influenza A virus and serologically belongs to Group 6 of the avian influenza viruses. The virus possesses the recognised biochemical and biophysical properties of influenza viruses. Experimental infection showed that the strain is not very pathogenic. It can be reisolated from the conjunctiva of experimentally infected birds. Résumé Isolement, caractérisation et propriétés biologiques d'un virus influenza A du groupe 6 trouvé chez des canards (Duck/Germany 1868/68) On isole un virus ARN hémagglutinant dans un troupeau de canetons brusquement atteints d'une maladie manifestant une forte mortalité (20%). Ce virus possède un antigène spécifique du groupe commun aux virus influenza A et peut ětre classé sérologiquement dans le groupe 6 des virus influenza aviaires. Il possède les propriétés biochimiques et biophysiques connues chez les virus de l'influenza. Lors d'une infection expérimentale, la souche isolée ne s'est pas révélée très pathogène. Le virus a pu ětre r'isolé dans les conjonctives des animaux infectés expérimentalement. Resumen Aislamiento, caracterización y propiedades biológicas de un virus influenza A del grupo 6 aislado en patos (Duck/Germany 1868/68) De una manada de patipollos, que enfermaron repentinamente con una cuota elevada de mortalidad (20%), se aisló un virus ARN hemoaglutinante, que posee un antígeno específico de grupo común a los virus influenza A y que se encuadra serológicamente en el grupo 6 de los virus influenza aviares. Posee las características bioquímicas y biofísicas conocidas de los virus influenza. En la infección experimental, la estirpe aislada no se mostró muy patógena. El virus se pudo reaislar de las conjuntivas de animales infectados experimentalmente.