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Carbonic anhydrase (CA) has been immobilized on surfactant-modified silylated chitosan (SMSC) for the carbonation reaction. CA immobilized on SMSC was characterized using a scanning electron microscope, energy-dispersive X-ray and X-ray diffraction spectroscopy, and Fourier transform infrared analysis. The effect of various parameters, such as pH, temperature, and storage stability, on immobilized CA was investigated using a p-nitrophenyl acetate (p-NPA) assay. The optimum pH and temperature were determined to be 7 and 35 °C, respectively. Kinetic parameters of immobilized and free CA (K m and V max values) were also evaluated. For immobilized CA, the K m value was 4.547 mM and the V max value was 1.018 mmol min -1 mg -1 , whereas for the free CA, the K m value was 1.211 mM and the V max value was 1.125 mmol min -1 mg -1 . It was observed that immobilized CA had longer storage stability and retained 50% of its initial activity up to 30 days. Proof of concept has been established for the biomimetic carbonation reaction. The CO 2 sequestration capacity in terms of conversion of CO 2 to carbonate was quantified by gas chromatography (GC). It was 10.73 and 14.92 mg of CaCO 3 /mg of CA for immobilized and free CA, respectively, under a limiting concentration of CO 2 (14.5 mg of CO 2 /10 mL).

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