Although bioluminescence (BL) in the open ocean has been extensively studied, coastal BL remains poorly understood due, in large degree, to a lack of BL instrumentation appropriate to measure the fine-scale biological and physical complexity of the coastal regime. As a contribution toward understanding coastal BL, we developed the Multipurpose Bioluminescence Bathyphotometer (MBBP). This compact, self-contained bathyphotometer (BP) was designed to function in a variety of deployment modes, including conventional shipboard profilers, towed platforms, autonomous underwater vehicles (AUVs), and profiling moorings. In all configurations, the instrument preserves signal structure at centimeter to meter scale resolution, the scale at which higher-flow instruments might disturb thin layers and other fine-scale water column features. In the MBBP, seawater is conveyed with minimal premeasurement excitation into a light-baffled stimulation and measurement chamber at a continuously measured flow rate of 350 to 400 mL s -1. A photomultiplier tube (PMT) records light from bioluminescent organisms after they are mechanically stimulated at the chamber entrance by a high-velocity impeller. Calibration and test protocols were developed to determine BL stimulation efficiency and MBBP measurement characteristics. To illustrate the capabilities of the MBBP to resolve the fine-scale structure of the BL community, measurements from two coastal environments are presented.
A new method is described employing small drone aircraft for antenna pattern measurements (APMs) of high-frequency (HF) oceanographic radars used for observing ocean surface currents. Previous studies have shown that accurate surface current measurements using HF radar require APMs. The APMs provide directional calibration of the receive antennas for direction-finding radars. In the absence of APMs, so-called ideal antenna patterns are assumed and these can differ substantially from measured patterns. Typically, APMs are obtained using small research vessels carrying radio signal sources or transponders in circular arcs around individual radar sites. This procedure is expensive because it requires seagoing technicians, a vessel, and other equipment necessary to support small-boat operations. Furthermore, adverse sea conditions and obstacles in the water can limit the ability of small vessels to conduct APMs. In contrast, it is shown that drone aircraft can successfully conduct APMs at much lower cost and in a broader range of sea states with comparable accuracy. Drone-based patterns can extend farther shoreward, since they are not affected by the surfzone, and thereby expand the range of bearings over which APMs are determined. This simplified process for obtaining APMs can lead to more frequent calibrations and improved surface current measurements.
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