The best co-digestion ratio between mezcal vinasses and bovine manure was investigated to produce methane.Five vinasses and bovine manure mixtures in different proportionswere compared (v/v): 25-75, 50-50, 75-25, 100-0, and 0-100, with six replications. As reactors, 496 mL glasscontainers with hermetic seal were used, with 300 mL of active volume under mesophilic conditions (36 ± 1 oC) for 10 days. The pH, total solids, and volatile solids of vinasses,bovine manure, and their mixtures were determined in triplicate.The methane accumulated volume was greater with the 0-100 and 75-25, followed by the 100-0, with 286.23, 286.40, and 225.48 NmL CH4, respectively. Methane yield was higher with 100-0 and 75-25 with 28.27 and 22.77 NmL CH4 g-1volatile solids, respectively. The Gompertz bacterial growthmodel showed that the micro-organisms adaptation periodin vinasses was longer than in bovine manure. Co-digestionof vinasses and bovine manure in proportion 75-25 % improvedthe methane production by 26.7 % with respect to the digestion of vinasses alone, and reduced the adaptation timeof bacteria to vinasses by 4.12 days.

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