Microbial degradation has long been recognized as the key rescue mechanism in shaping the oil polluted marine environments and the role of indigenous populations or their functional genomics have never been explored from Indian marine environments, post an oil spill event. In the current study, high throughput metagenomic analysis, PLFA profiling and mass spectrophotometric analysis was performed in combination with metabolomics to capture signature variations among the microbial communities in sediment, water and laboratory enrichments. Contrary to the previous reports, the bloom of Pseudomonadales (specifically genus Acinetobacter) in oiled sediment and Methylococcales in oiled water outnumbered the relative abundance of Alcanivorax in response to hydrocarbon contamination. Overall enhancement of xenobiotic degradation was suggested by metabolomic analysis in sediment and water post the spill event and varying quantitative assemblage of enzymes were found to be involved in hydrocarbon utilization. Laboratory enrichments revealed the competitive advantage of sediment communities over the water communities although unique taxa belonging to the later were also found to be enriched under in vitro conditions. Simultaneous analysis of sediment and water in the study provided explicit evidences on existence of differential microbial community dynamics, offering insight into possibilities of formulating nature identical solutions for hydrocarbon pollution.

Isolation and characterization of heterotrophic Gram-negative bacteria was carried out from the sediment and water samples collected from Kongsfjord, Arctic. In this study, the potential of Arctic bacteria to tolerate heavy metals that are of ecological significance to the Arctic (selenium (Se), mercury (Hg), cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn)) was investigated. Quantitative assay of 130 isolates by means of plate diffusion and tube dilution methods was carried out by incorporation of different concentrations of metals. Growth in Se and Pb at a concentration of 3000 μg/L was significantly lower (P≤0.0001) than at 2000 μg/L. The minimum inhibitory concentration for Cd and Hg was 50 μg/L (P≤0.0001, F=264.23 and P≤0.0001, F=291.08, respectively) even though in the tube dilution test, Hg-containing tubes showed much less growth, revealing its superior toxicity to Cd. Thus, the level of toxicity of heavy metals was found to be in the order of Hg>Cd>Cu>Zn>Pb>Se. Multiple-metal-resistant isolates were investigated for their resistance against antibiotics, and a positive correlation was observed between antibiotic and metal resistance for all the isolates tested. The resistant organisms thus observed might influence the organic and inorganic cycles in the Arctic and affect the ecosystem.

Anthropogenic activities experienced a pause due to the nationwide lockdown, imposed to contain the rapid spread of COVID-19 in the third week of March 2020. The impacts of suspension of industrial activities, vehicular transport and other businesses for three months (25 March-30 June) on the environmental settings of Chennai, a coastal megacity was assessed. A significant reduction in the key urban air pollutants [PM 2.5 (66.5%), PM 10 (39.5%), NO 2 (94.1%), CO (29%), O 3 (45.3%)] was recorded as an immediate consequence of the reduced anthropogenic activities. Comparison of water quality of an urban river Adyar, between pre-lockdown and lockdown, showed a substantial drop in the dissolved inorganic N (47%) and suspended particulate matter (41%) during the latter period. During the pandemic, biomedical wastes in India showed an overall surge of 17%, which were predominantly plastic. FTIR-ATR analysis confirmed the polymers such as polypropylene (25.4%) and polyester (15.4%) in the personal protective equipment.

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