Utilization of agro-industrial wastes for the production of lipase from Stenotrophomonas maltophilia isolated from Arctic and optimization of physical parameters

Neethu, C.S.; Rahiman, K M Mujeeb; Rosmine, Emilda; Saramma, A. V.; Hatha, A. A. Mohamed

doi:10.1016/j.bcab.2015.09.002

Cited by 26 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The active fractions were then pooled and electrophoretic mobility of the fractions was analysed by SDS-PAGE. The monomeric band was found to be approximately 23 KDa which was identical to the enzyme reported from Streptomyces cyaneus SN32 [20] and Streptomyces sp. SKK1-8 [14].…”

Section: Resultssupporting

confidence: 80%

“…The marine environment contains complex polysaccharides (CP) derived from terrestrial and aquatic sources; hence, it is a rich resource for microorganisms equipped with the enzymes series for CP degradation [17], [18]. Furthermore, actinomycetes are a microbial group not fully exploited for hydrolytic enzyme production despite their record of expansive metabolite production [19], [20]. Streptomyces species are notable producers of several highly active secondary metabolites and extracellular enzymes [22], [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Statistical optimisation of xylanase production by estuarine Streptomyces sp. and its application in clarification of fruit juice

Rosmine

Sainjan

Silvester

et al. 2017

Journal of Genetic Engineering and Biotechnology

Self Cite

View full text Add to dashboard Cite

Recently, xylanase has become an essential option for environmental friendly industrial biotechnological applications and the rising demand for its large scale production urge to take the advantage of statistical approach of optimization to investigate the interactive effects of prominent process factors involved to enhance xylanase production. In the present study, xylanase production from Streptomyces sp. strain ER1 isolated from Cochin estuarine soil; was optimised using statistical designs- Plackett-Burman and Central composite design. Plackett-Burman design was used to identify important fermentation condition factors affecting the xylanase production using beechwood xylan as the substrate. The optimum levels of these significant factors were determined employing the Central Composite Design. Out of the thirteen factors screened, concentration of beechwood xylan and olive oil, agitation speed, and inoculum age were recognized as the most significant factors. By analyzing the response surface plots and using numerical optimization method, the optimal levels for concentration of xylan and olive oil, agitation speed and inoculum age were determined as 0.37%, 33.10 mg/L, 42.87 RPM and 21.05 h, respectively. The optimised medium resulted in a 1.56-fold increased level of the xylanase (10,220 U/mL) production compared to the initial level (3986.444 U/mL) after 120 h of fermentation. The purified enzyme could successfully clarify orange, mousambi and pineapple juice to 20.87%, 23.64% and 27.89% respectively. Thus the present study has proved that Streptomyces sp. strain ER1 (KY449279) is a potential and useful organism for xylanase production and its purified enzyme could clarify the selected fruit juices.

show abstract

Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Statistical optimisation of xylanase production by estuarine Streptomyces sp. and its application in clarification of fruit juice

Rosmine

Sainjan

Silvester

et al. 2017

Journal of Genetic Engineering and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lipases constitute one of the most important industrial enzymes, and are used in various industrial and scientific processes that require hydrolysis, esterification, transesterification, and alcoholysis 11 – 13 . Due to substrate specificity, regio-, and enantioselectivity, lipases are, indeed, widely used to manufacture food, pharmaceuticals, cosmetics, detergents, and paper 14 – 16 . Proteases are similarly valuable commercially, and are widely used at industrial scale to produce detergent, pharmaceuticals, leather, and silk.…”

Section: Introductionmentioning

confidence: 99%

Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Boratyński

Szczepańska

Grudniewska

et al. 2018

Sci Rep

View full text Add to dashboard Cite

The study show usefulness of rapeseed cake, rich in fats and proteins byproduct generated after oil production, which may be used as a microbial medium for lipase and protease biosynthesis. Of 26 different filamentous fungi screened by solid-state fermentation, Penicillium camemberti AM83 was found to abundantly produce lipase and protease. Various process parameters were then optimized to maximize lipase and protease secretion, including carbon and nitrogen source, C/N ratio, metal ions, temperature, moisture content, initial pH, and inoculum size. Lipase production increased approximately 11.2-fold in solid-state cultures on rapeseed cake supplemented with lactose and calcium chloride, alkalinized to pH 8, hydrated to 80%, and inoculated with 1.2 × 106 spores/mL. Similarly, protease production increased approximately 8.4-fold in optimized cultures inoculated with 3.2 × 108 spores/mL, and grown on rapeseed cake with lactose and ammonium sulfate at pH 9 and moisture content 60%. The results highlight the potential economic value of solid-state fermentation on rapeseed cake to produce industrial hydrolases.

show abstract

“…The oil extraction process generates a sub-product with a high protein content (59%) (Wang et al, 2018), which represents between 60 and 75% of the seed's total weight (V asquez-Osorio et al, 2017). Currently, these sub-products are not effectively used in production systems (Dai and Huang, 2016) that cause environmental problems due to their easy decomposition (Neethu et al 2015). The scientific literature does not report the use of this sub-product as a fermentation substrate.…”

Section: Introductionmentioning

confidence: 99%

“…In the bioprocessing industry, there is interest in the use of residual materials due to their easy accessibility and the C and N 2 content, necessary elements in fermentation processes (Neethu et al 2015); however, the greatest interest is the possibility of reducing the costs associated with the substrate, given that these constitute between 60 and 70% of the total cost of the process (Khedkar et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Formulation of a fermentation substrate from pineapple and sacha inchi wastes to grow Weissella cibaria

MICANQUER-CARLOSAMA

Cortés

Serna-Cock

2020

Heliyon

View full text Add to dashboard Cite

Gold honey variety pineapple wastes and sacha inchi sub-products (SIS) were characterized in their elemental, physical, and chemical form in order to formulate a supplemented fermentation substrate (SFS) for the growth Weissella cibaria. The peels and fresh cores of the pineapple (FPP, FPC) were dried and ground (PPP, PPC) and then mixed (MCPP). The following procedures were then undertaken: a physicochemical characterization (moisture, a w , pH, acidity, and soluble solids) of the SIS, FPP, FPC, PPP, and PPC; a proximal characterization of he FPP, FPC, SIS, and SFS; and an elemental analysis (C-N 2-H 2-O 2-S) of the MCPP, SIS, and W. cibaria, which allowed the stoichiometric equation to be defined and the SFS to be formulated. We then evaluated the effect that homogenization and heating to boiling point had on the concentration of reducing sugars in the SFS (g L À1). Finally, W. cibarias kinetic fermentation parameters were evaluated in the SFS and in a commercial substrate (control). The results showed FPP and FPC yields of 26.02 AE 0.58 and 14.69 AE 1.13%, respectively; a higher total sugar content in FPC (7.21%) than in FPP (6.65%); a high crude protein content in SIS (56.70%), and a C:N 2 ratio of 6.50:1.00. Moreover, the highest concentration of reducing sugars (4.44 AE 0.29 g L À1) in the SFS was obtained with 5 h of hydrolysis under homogenization pre-treatments and heating until boiling. The SFS allowed the adaptation of W. cibaria, and there was a biomass production of 2.93 g L À1 and a viability of 9.88 log CFU mL À1. The formulation of an unconventional fermentation substrate from-Agro-industrial wastes of pineapple and sacha inchi to produce valuable products (such as lactic acid biomass through fermentation), is an excellent perspective for large-scale application.

show abstract

Utilization of agro-industrial wastes for the production of lipase from Stenotrophomonas maltophilia isolated from Arctic and optimization of physical parameters

Cited by 26 publications

References 26 publications

Statistical optimisation of xylanase production by estuarine Streptomyces sp. and its application in clarification of fruit juice

Statistical optimisation of xylanase production by estuarine Streptomyces sp. and its application in clarification of fruit juice

Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Formulation of a fermentation substrate from pineapple and sacha inchi wastes to grow Weissella cibaria

Contact Info

Product

Resources

About