No abstract

SummaryAscorbate peroxidases (APX), localized in the cytosol, peroxisomes, mitochondria and chloroplasts of plant cells, catalyze the reduction of H 2 O 2 to water by using ascorbic acid (ASA) as speci®c electron donor. The chloroplastic isoenzymes of APX are involved in the water±water cycle, which contributes to the photophosphorylation coupled to the photosynthetic electron transport. In order to better clarify the contribution of thylakoidal APX (tAPX) to the reactive oxygen species (ROS) scavenging activity, as well as to the ®ne modulation of ROS for signaling, we produced Arabidopsis lines overexpressing tAPX. These lines show an increased resistance to treatment with the O 2 À generating herbicide Paraquat (Pq). However, when challenged with photoinhibitory treatments at high light or low temperature, or with iron (Fe) or copper (Cu) overload, the tAPX-overexpressing lines show no increased resistance with respect to controls, indicating that in such experimental conditions, tAPX overexpression does not reinforce plant defenses against the oxidative stresses tested. Interestingly, the nitric oxide (NO)±donor sodium nitroprusside (SNP) represses accumulation of tAPX transcript; SNP also partially inhibits tAPX enzymatic activity. After treatment with SNP, the tAPX-overexpressing lines show reduced symptoms of damage with respect to control plants treated with SNP. These transgenic lines con®rm that H 2 O 2 acts in partnership with NO in causing cell death and highlight the important role of tAPX in the ®ne modulation of H 2 O 2 for signaling.

SummaryNitric oxide (NO) is a signaling molecule that plays a critical role in the activation of innate immune and in¯ammatory responses in animals. During the last few years, NO has also been detected in several plant species and the increasing number of reports on its function in plants have implicated NO as an important effector of growth, development and defense. Analogously to animals, NO has been recently shown to inhibit tobacco aconitase. This suggests that NO may elevate free iron levels in the cells by converting tobacco cytoplasmic aconitase into a mRNA binding protein that negatively regulates accumulation of ferritin.We investigated the possible role of NO as a regulator of ferritin levels in Arabidopsis and found that the NO-donor sodium nitroprusside (SNP) induces accumulation of ferritin both at mRNA and protein level. Iron is not necessary for this NO-mediated ferritin transcript accumulation, since SNP is still able to induce the accumulation of ferritin transcript in Arabidopsis suspension cultures pre-treated with the iron chelants DFO or ferrozine. However, NO is required for iron-induced ferritin accumulation, as the NO scavenger CPTIO prevents ferritin transcript accumulation in Arabidopsis suspension cultures treated with iron. The pathway is ser/thr phosphatase-dependent and necessitates protein synthesis; furthermore, NO mediates ferritin regulation through the IDRS sequence of the Atfer1 promoter responsible for transcriptional repression under low iron supply.NO, by acting downstream of iron in the induction of ferritin transcript accumulation is therefore a key signaling molecule for regulation of iron homeostasis in plants.

The analysis of the data collected by the NA50 experiment in 1998, reported in this paper, extends and clarifies the pattern of the previously observed J/ψ anomalous suppression. This new measurement, besides providing a deeper understanding of the previous observations, reveals a steady significative decrease in the J/ψ production rate up to the most central Pb-Pb collisions. It clearly rules out the presently available conventional (hadronic) models of J/ψ suppression, which unanimously predict a saturation of the J/ψ rate for central Pb-Pb collisions. On the contrary and together with the sharp onset of the anomalous suppression previously reported, the new observation leads to a global production rate pattern which finds its natural explanation in the framework of the formation of a deconfined state of quarks and gluons.

Giant reed (Arundo donax) is a promising energy crop of the Mediterranean areas. It has long been associated with humans and has been cultivated in Asia, southern Europe, North Africa and the Middle East for thousands of years. It is a perennial herbaceous plant (Poaceae) found in grasslands and wetlands throughout a wide range of climatic zones. Amplified fragment length polymorphism (AFLP) analysis was used to assess genetic inter and intrarelationships between A. donax and other Arundo species. Furthermore, the development of the sexual apparatus was analysed to understand the basis of sterility in the accession examined. The dendrograms obtained by phenetic and cladistic analysis support the monophyletic origin of giant reed and suggest that it originated in Asia and began to spread into the Mediterranean without traces of hybridisation with the other Arundo species. In particular, samples from Mediterranean areas are characterisd by a lower gene diversity and incidence of rare AFLP fragments indicating that these populations are recent in origin. Moreover, results indicate the occurrence of post-meiotic alterations in the ovule and pollen developmental pathway. Thus, the success of giant reed can be attributed mainly to its rapid clonal spread by rhizome extension, flood dispersal of rhizome and culm fragments.