Background2-phenylethanl (2-PE) and its derivatives are important chemicals, which are widely used in food materials and fine chemical industries and polymers and it’s also a potentially valuable alcohol for next-generation biofuel. However, the biosynthesis of 2-PE are mainly biotransformed from phenylalanine, the price of which barred the production. Therefore, it is necessary to seek more sustainable technologies for 2-PE production.ResultsA new strain which produces 2-PE through the phenylpyruvate pathway was isolated and identified as Enterobacter sp. CGMCC 5087. The strain is able to use renewable monosaccharide as the carbon source and NH4Cl as the nitrogen source to produce 2-PE. Two genes of rate-limiting enzymes, chorismate mutase p-prephenate dehydratase (PheA) and 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase (DAHP), were cloned from Escherichia coli and overexpressed in E. sp. CGMCC 5087. The engineered E. sp. CGMCC 5087 produces 334.9 mg L-1 2-PE in 12 h, which is 3.26 times as high as the wild strain.ConclusionsThe phenylpyruvate pathway and the substrate specificity of 2-keto-acid decarboxylase towards phenylpyruvate were found in E. sp. CGMCC 5087. Combined with the low-cost monosaccharide as the substrate, the finding provides a novel and potential way for 2-PE production.

The heliocentric orbits of STEREO A and B with a separation in longitude increasing by about 45°per year provide the unique opportunity to study the evolution of the heliospheric plasma sheet (HPS) on a time scale of up to~2 days and to investigate the relative locations of HPSs and heliospheric current sheets (HCSs). Previous work usually determined the HCS locations based only on the interplanetary magnetic field. A recent study showed that a HCS can be taken as a global structure only when it matches with a sector boundary (SB). Using magnetic field and suprathermal electron data, it was also shown that the relative location of HCS and SB can be classified into five different types of configurations. However, only for two out of these five configurations, the HCS and SB are located at the same position and only these will therefore be used for our study of the HCS/HPS relative location. We find that out of 37 SBs in our data set, there are 10 suitable HPS/HCS event pairs. We find that an HPS can either straddle or border the related HCS. Comparing the corresponding HPS observations between STEREO A and B, we find that the relative HCS/HPS locations are mostly similar. In addition, the time difference of the HPSs observations between STEREO A and B match well with the predicted time delay for the solar wind coming out of a similar region of the Sun. We therefore conclude that HPSs are stationary structures originating at the Sun.

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