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Objective:The objective of this study is to characterize the phytoconstituents of Tiliacora racemosa Colebr. using gas chromatography mass spectrometry (GC-MS).Methods: Preliminary phytochemical and physicochemical analysis was carried out using standard procedures. GC-MS analysis of methanolic extract was carried out using Thermo GC-Trace Ultra version: 5.0, Thermo MS DSQ with a DB 35MS capillary standard non-polar column and gas chromatograph interfaced to a mass selective detector (MS DSQ II) with Xcalibur software.Results: Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, triterpenoids, steroids, proteins and amino acids, carbohydrates, saponins and coumarin. Quinones, anthraquinones, glycosides and fixed oil were absent. GC-MS analysis revealed the presence of 28 compounds of which quinic acid (retention times [RT]: 15.65) and inositol, 1-deoxy-(CAS) (RT: 19.24) was observed as abundant compounds. Conclusion:The presence of various bioactive compounds confirms the medicinal importance and it's application for curing various diseases by traditional practitioners. However, isolation and characterization of potential bioactive compounds would lead to drug formulation.

Tiliacora racemosa is a climbing shrub belonging to the family Menispermaceae and is used in traditional medicine for treating different ailments such as snakebites, cuts and wounds, strangury and also used as a diuretic. The morphoanatomical studies help to identify diagnostic features for the identification and standardization of the medicinal plant. The plant material was fixed, sectioned using rotary microtome and stained with toluidine blue. The leaf consists of very thick midrib with small lateral veins both being plano-convex. The midrib consists of three top or egg shaped collateral vascular bundles which are surrounded by a common sclerenchymatous bundle sheath and the lamina is dorsiventral. The adaxial epidermis is apostomatic and the abaxial epidermis is stomatiferous which is of cyclocytic type. The stem is circular in sectional view and consists of about 19 discrete wedge shaped vascular bundles. The narrow and wide fibres, vessel elements were seen in powder microscopy. Wide fibres, vessel elements were seen in powder microscopy.

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