PurposeThe aim of this study was to establish the concurrent validity and reliability of four different two-dimensional (2D) video-based techniques for quantifying frontal plane knee kinematics during a 40 cm double-legged drop jump.Participants and methodsA convenience sample of 16 healthy participants (nine males and seven females; age: [mean ± standard deviation] 25.5±2 years; body mass index: 24.33±2.98 kg/m2) participated in this investigation. A total of five trials during a 40 cm drop jump maneuver with a countermovement jump were used as the functional task. Four knee valgus measures, such as two different frontal plane projection angle measures, knee-to-ankle separation ratio (KASR), and knee separation distance (KSD), were measured using 2D and three-dimensional (3D) systems. To generalize to the greater population of possible evaluators, the testers performing the biomechanical analyses were three novice physical therapists. Intra- and intertester intraclass correlation coefficients (ICCs) were estimated for 2D analysis variables. ICCs were estimated for all measures between systems to determine concurrent validity of the 2D system.ResultsAll four 2D measures showed good to excellent reliability (ICC: 0.89–0.99). KASR and KSD showed excellent correlation (ICC: 0.96; 95% CI: 0.82–0.98 and ICC: 0.94; 95% CI: 0.90–0.96, respectively) with the 3D system, while both methods of frontal plane projection angle showed poor to moderate correlation (ICC: 0–0.57) with the 3D system.Conclusion2D KASR and KSD measures are cost effective, reliable, and highly correlated with the same measures using 3D techniques for the evaluation of knee valgus.

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Zebrafish is becoming a popular animal model in neuropharmacology and drug discovery, mainly due to its ease of handling and low costs involved in maintenance and experimental work. This animal displays a series of complex behaviours that makes it useful for assessing the effects of psychoactive drugs. Here, adult zebrafish were used for assessment of the anxiolytic and anti-addictive properties of UFR2709, a nicotinic receptor (nAChR) antagonist, using two behavioural paradigms to test for addiction, the novel tank diving test to assess anxiety and the conditioned place preference (CPP). Furthermore, the expression of nAChR subunits α4 and α7 was measured in the zebrafish brain. The results show that UFR2709 exhibits an anxiolytic effect on zebrafish and blocks the effect evoked by nicotine on CPP. Moreover, UFR2709 significantly decreased the expression of α4 nicotinic receptor subunit. This indicates that UFR2709 might be a useful drug for the treatment of nicotine addiction.

Introducción. El teletrabajo producto del COVID-19 ha generado exigencias que podrían promover la generación de un sobreesfuerzo vocal. Objetivo. Evaluar cómo las condiciones de trabajo pueden afectar la salud vocal, evaluar el estrés laboral y valorar los cambios en la calidad vocal de profesoras de primaria de la comuna de Temuco. Método. Se evaluó a 18 profesoras de primaria de la comuna de Temuco, Chile, quienes se encontraban trabajando en modalidad de teletrabajo durante el año 2021, realizando una evaluación al comienzo y al final del año escolar, a través del Voice Handicap Index (VHI-30), Vocal Tract Disconfort (VTD), Maslach Burnout Inventory (MBI) y análisis acústico mediante medidas de perturbación y espectro promedio a largo plazo. Resultados. Solo el 21% de las profesoras contaba con un espacio físico adecuado para el teletrabajo. El 52,6% debía compartir responsabilidades del hogar, como el cuidado de hijos menores durante su jornada de teletrabajo. Los resultados muestran un aumento de la sintomatología y sensaciones de incomodidad a nivel del tracto vocal medidas mediante el VHI-30 y el VTD. En cuanto al estrés laboral medido por la escala de Burnout, solo se evidenció un valor significativo para la subescala agotamiento emocional. No se evidenciaron diferencias significativas en los parámetros acústicos Jitter, Shimmer, HNR y Alpha ratio. Conclusiones. La gran mayoría de las participantes manifestó un aumento del malestar y sintomatología vocal autopercibida (sequedad de garganta, fatiga vocal) en conjunto con un mayor nivel de estrés, lo que a la larga puede desencadenar trastornos de voz.