No abstract

Rubus glaucus Benth (known as “mora de castilla”) is a Colombian agricultural product, with probably, the major potential. This fruit combines features of Idaeobatus and Rubus subgenera. Despite its recognized importance in the economy of small producers, this crop has received little technological development; as a result, sowing procedures of this specie is done by using local cultivars asexually propagated by producers. Associated Rubus producers in Colombia has noticed the necessity to formalize the offer of planting material, starting with plant breeding programs tending to the obtention of more productive varieties with morphological features that facilitates cultural activities. This study presents the results of the evaluation of SSR and SNPs markers obtained in previous experimental works from a RNA-Seq transcriptome analysis. It was evaluated 15 promissory R. glaucus cultivars, which could be potential progenitors in future breeding schemes. Genetic characterization was accomplished by testing 22 SSR microsatellite and 78 single nucleotide polymorphisms (SNPs) markers. From evaluated SSR markers, 15 yielded positive PCR amplification generating 29 loci and 58 alleles. From evaluated SNPs markers, 36 yielded positive PCR amplification. Obtained sequences from amplified products with SNPs showed high homology with species belonging to Rosaceae family. Selection criteria of progenitors were based on the results of molecular characterizations and useful morphological features in the culture management. This research demonstrates the utility of molecular markers to assess genetic diversity of potential progenitors susceptible to plant breeding processes. This process, highly known as development of parental populations, determines in a great manner the success of plant breeding processes.

The low annual growth rate of the stipe in oil palm progenies is desirable to increase these crops’ productive and economic life. Recurrent reciprocal selection (R.R.S.) has allowed the development of oil palm populations through several breeding cycles with an increased frequency of favorable alleles associated with traits of interest. The present study evaluated families derived from Deli dura × African dura crosses. For 12 years, the yield, vegetative characteristics, and the amount of oil in seven dura progenies were assessed to estimate, from the information collected, the genetic parameters, heritability, and phenotypic correlations among quantitative genetic traits of high-yielding dwarf progenies. The analysis was carried out using analysis of variance, followed by a comparison of means for all estimated traits. The effect of the progenies was highly significant (p ≤ 0.01) for most traits. The yield values, expressed in fresh fruit bunches (FFB) for the progenies, ranged from 165 to 208 kg per palm per year. The oil-to-bunch ratio (O/B) ranged from 17% to 19%, with an overall average of 18%. One of the essential characteristics in this study was the vertical growth of the stipe. Progenies P6 and P7 were identified as those with the lowest annual increase in height, with values of 0.29 and 0.33 m year−1. The values indicate that these are slow-growing cultivars with a high FFB yield and O/B. The highest heritabilities were found for the vegetative trait height (71.62%) and the number of leaflets (46.64%). The development of dura parents with slow growth characteristics in combination with a high bunch and oil production allows extending the productive life of the crop to more than 35 years, providing added value to obtaining differentiated cultivars of oil palm.

Fue empleado el método REML/BLUP para estimar los parámetros genéticos, y seleccionar los mejores individuos provenientes de una población de hermanos germanos de Dura x Dura, a partir de un análisis de correlación entre caracteres, realizando un agrupamiento de familias por disimilaridad multivariada y determinación del número de medidas repetidas necesarias para la selección de las características (número y peso medio de racimos). Fueron evaluadas 24 familias procedentes de tres ensayos del banco de germoplasma de la estación experimental Santo Domingo del INIAP en Ecuador. La evaluación fue realizada en un periodo de cinco años, empleando un diseño en bloques al azar, con doce plantas por parcela y cuatro repeticiones. La variabilidad de la población en relación a las características evaluadas y heredabilidad de individuos dentro del bloque, similar a la encontrada dentro de familias en las parcelas. La ganancia genética de las 10 plantas seleccionadas representa un 43% superior a la media general. La correlación fue baja y negativa para número de racimos y peso medio de racimos. Con base en el agrupamiento de Tocher se obtuvieron seis grupos, donde el grupo IV agrupa las familias seleccionadas por el Rank-medio multivariado (3A, 5C y 7B). Se puede concluir que las estimativas obtenidas por el BLUP, estimulan la continuidad del programa de mejoramiento genético de racimos, con posibilidad de maximizar las ganancias genéticas en generaciones futuras.

Resumo -O objetivo deste trabalho foi desenvolver um processo de integração de mapas genéticos, com o uso do inverso da variância, e testar sua eficiência. Foram utilizadas populações simuladas F 2 codominante e de retrocruzamento, com tamanhos populacionais de 100, 150, 200 e 400 indivíduos, tendo-se considerado uma espécie diploide fictícia com 2n = 2x = 2 cromossomos, com o comprimento total do genoma por grupo de ligação estipulado em 100 cM, 21 marcas por grupo de ligação e marcadores equidistantes em 5 cM. Os genomas foram comparados quanto ao tamanho do grupo de ligação, variância das distâncias entre marcas adjacentes, correlação de Spearman e quanto ao estresse relativo à adequação das distâncias estimadas. Cada genoma simulado foi fragmentado em quatro novos mapas: três com oito marcadores e um com nove marcadores, cada qual com quatro marcadores âncoras. Os mapas foram alinhados, ordenados, integrados e, em seguida, comparados ao mapa de origem. O processo de integração de mapas proposto mostrou-se eficiente. Os mapas gerados tiveram pequena tensão interna em comparação aos mapas dos quais se originaram. A integração de mapas depende do tipo de população utilizada, tamanho da população, tipo de marcador, da frequência de recombinação e da fase de ligação.Termos para indexação: frequência de recombinação, grupos de ligação, marcador molecular, tensão interna. Variance utilization as an alternative methodology for integrating genetic mapsAbstract -The objective of this work was to develop an integration process of genetic maps using the inverse variance, and to test its efficiency. Simulated F 2 codominant populations and backcrosses of 100, 150, 200, and 400 plants were used, considering a virtual diploid species with 2n = 2x = 2 chromosomes, with total genome length per linkage group estipulated in 100 cM, 21 marks per linkage group, and equidistant markers spaced at 5 cM. The genomes were compared for linkage group size, distance variance between adjacent marks, Spearman correlation and stress related to the estimated distances adequation. Each simulated genome was fragmented into four new maps: three with eight makers and one with nine, each containing four anchor markers. The maps were aligned, ordered, integrated and, then, compared with the original map. The proposed map integration process proved to be effective. The generated maps showed a little internal tension in comparison to the original ones. Map integration depends on the population type, population size, marker type, recombination frequency, and on the linkage phase.