RESUMO:O presente estudo teve como objetivo acompanhar a dinâmica folicular de dez éguas submetidas à inserção do dispositivo de liberação lenta de progesterona e, verificar se este procedimento antecipa o início da ciclicidade ovariana em éguas em anestro. Para isso, foram colhidos dados do uso de implante intravaginal de progesterona em éguas que se encontravam em anestro. Após dez dias da colocação do implante, o mesmo foi removido e administrado 0,25 mg de prostaglandina F2α por via intramuscular. As éguas foram diariamente acompanhadas, por meio de exame ultrassonográfico, até o folículo ovariano apresentar-se com 35 mm de diâmetro para se administrar hCG e GnRH e, no dia seguinte, realizar-se a inseminação artificial e aguardar a ovulação. Oito dias após a ovulação foi realizada a coleta dos embriões. Dentro das condições do presente experimento, concluímos que a utilização do implante intravaginal de progesterona sobre a ciclicidade das éguas em anestro demonstrou 80% de eficácia, conseguindo-se 75% de embriões viáveis. Podemos concluir, também, que a faixa etária das éguas não influenciou a eficácia do implante, já que éguas com idade entre três e 25 anos foram responsivas ao uso do implante intravaginal de progesterona.Palavras-chave: Éguas. Ultrassonografia. Reprodução. hCG. GnRH. EFFECT OF INTRAVAGINAL PROGESTERONE IMPLANT CYCLICALITY ON OF MARES IN ANESTROUS QUARTER MILE RACE SUMMARY:The purpose of the present paper was following up the follicular dynamic of ten mares submitted to the insertion of the device for slow liberation of progesterone and, knowing whether or not it accelerated the beginning of the ovarian cyclicity in mares in anestrus. For that reason, were collected data about the use of progesterone intravaginal implantation were in anestrus. 10 days after the implantation placement, the same was removed and it was administered prostaglandin F2α. The mares were followed up through US till the follicles were 35 mm of diameter to administer human chorionic gonadotropin (hCG), in order to, on the following day, accomplishing the IA and waiting for ovulation. Then, after eight days it was accomplished the embryos collection. Within the conditions of the present experiment, we've concluded that using the progesterone intravaginal implantation on the cyclicity of mares in anestrus has demonstrated 80% of efficacy, making it possible obtaining 75% of viable embryos. We can conclude also that, the mares age group hasn't influenced the implantation efficacy, since the mares between 3 and 25 years old were responsive to the use of progesterone anestrus.
O presente estudo teve como objetivo acompanhar a dinâmica folicular de dez éguas submetidas à inserção do dispositivo de liberação lenta de progesterona e, verificar se este procedimento antecipa o início da ciclicidade ovariana em éguas em anestro. Para isso, foram colhidos dados do uso de implante intravaginal de progesterona em éguas que se encontravam em anestro. Após dez dias da colocação do implante, o mesmo foi removido e administrado 0,25 mg de prostaglandina F2α por via intramuscular. As éguas foram diariamente acompanhadas, por meio de exame ultrassonográfico, até o folículo ovariano apresentar-se com 35 mm de diâmetro para se administrar hCG e GnRH e, no dia seguinte, realizar-se a inseminação artificial e aguardar a ovulação. Oito dias após a ovulação foi realizada a coleta dos embriões. Dentro das condições do presente experimento, concluímos que a utilização do implante intravaginal de progesterona sobre a ciclicidade das éguas em anestro demonstrou 80% de eficácia, conseguindo-se 75% de embriões viáveis. Podemos concluir, também, que a faixa etária das éguas não influenciou a eficácia do implante, já que éguas com idade entre três e 25 anos foram responsivas ao uso do implante intravaginal de progesterona.
The mare being a seasonal polyestrous animal is a limiting factor for maintenance of embryo transfer programs during the entire year.At the beginning and end of the breeding season, the percentage of recipients showing normal estrous cycles may be low compared to donors. Exogenous progesterone has been administered to acyclic mares to allow them to be used as recipients. Noncyclic recipient mares treated once a week with long-acting progesterone (P4) have been widely used in Brazil in equine embryo transfer (ET) programs. P4LA was developed for weekly administration of 1.5 g of P4 to acyclic recipients until approximately 120 days of pregnancy. The aim of the present experiment was to evaluate the pregnancy rates of noncyclic recipients treated with a new formulation of long-acting P4 developed in our laboratory that allows a longer interval between treatments (14 days). Noncyclic mares (n = 36) were used as recipients between August and September of 2008 (transition between winter and spring in Brazil). Noncyclic mares were treated with 5 mg of estradiol benzoate during 2 consecutive days followed by the application of 10 mL (i.m.) of P4LA containing 300 mg mL-1 of P4, for a total P4 dose of 3 g. Embryos were transferred between 6 to 12 days after P4LA injection. The same dose of P4LA was repeated on the day of ET and every 14 days until 110 days after pregnancy diagnosis. One hundred fifty-five cyclic recipients were used as a control group, with embryos transferred 4 to 8 days after ovulation. No differences were observed (P > 0.05) when comparing pregnancy rates from acyclic recipients treated with P4LA (82%, 56/68) or cyclic recipients (88%; 137/155) on Day 15. The rate of embryonic loss at 50 days was similar (P > 0.05) for noncycling (11%) and cycling recipient mares (10%). The results obtained in the present study demonstrated that the new formulation of P4LA containing 300 mg mL-1 of P4 and administered every 14 days was effective in maintaining pregnancy in noncyclic recipient mares, allowing a larger treatment interval and longer window of time to transfer embryos.
Relação do proteinograma sérico e as fases folicular e luteal do ciclo estral em éguas* Relation of the serum proteins and the follicular and luteal phases of the estrous cycle in mares
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