SummaryThe mitochondrial electron transport chain (ETC) includes an alternative oxidase (AOX) that may control the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). ROS and RNS act as signaling intermediates in numerous plant processes, including stomatal movement.The role of AOX in controlling ROS and RNS concentrations under both steady-state and different stress conditions was evaluated using Nicotiana tabacum plants lacking AOX as a result of RNA interference. A potential functional implication of changes in ROS and RNS homeostasis was also evaluated by examining stomatal function.The leaves of nonstressed AOX knockdowns maintained concentrations of H 2 O 2 and nitric oxide (NO) normally seen in wildtype plants only under stress conditions. Further, guard cell NO amounts were much higher in knockdowns. These guard cells were altered in size and were less responsive to NO as a signal for stomatal closure. This, in turn, compromised the stomatal response to changing irradiance.The results reveal a role for AOX in stomata. A working model is that guard cell AOX respiration maintains NO homeostasis by preventing over-reduction of the ETC, particularly during periods when high concentrations of NO acting as a signal for stomatal closure may also be inhibiting cyt oxidase respiration.

During solid surface impact, a falling drop's energy is transformed into oscillations of its liquid/gas interface. We consider drop deposition during oblique impact in the capillary-ballistic regime characterized by high Reynolds number and moderate Weber number. We treat this as an inverse problem showing that post-impact observations of the frequency spectrum and modal partition of energy allow one to determine a drop's pre-impact characteristics and wetting properties. Our analysis is useful for quantifying contact-line dissipation during inertial spreading and can be used as a diagnostic technique for determining substrate wetting properties.