The objective of this study was to perform phytochemical screening, to determine the content of phenolic compound, to evaluate antioxidant, anti-inflammatory and antiangiogenic activities of ethanol, waterethanol and water extracts of Lophira procera. Antioxidant activity was determined by 2,2-diphenyl-1picrylhydrazyl (DPPH) and phosphomolybdenum assay, anti-inflammatory activity by proteins denaturation inhibition and membranes stabilization test and antiangiogenic activity by chicken chorioallantoic membrane (CAM) method. The results showed that this plant is rich in saponins, polyphenols, tannins, total flavonoids, proanthocyanidins and coumarins. Extracts presented a strong antioxidant activity (IC 50 values of 5.452 ± 0.119 lg/mL and 6.346 ± 0.544 lg/mL and respective AAI of 9.173 ± 0.203 and 7. 919 ± 0.711). Excellent anti-inflammatory activity was also observed (IC 50 = 16.952 ± 1.897 and IC 50 = 2 3.172 ± 0.066 lg/mL for inhibition of protein denaturation and membrane stabilization respectively). Finally, extracts manifested a very good anti-angiogenic activity (with inhibitions ranging from 57.142 ± 0.124% to 100%). These biological activities are certainly due to high content of phenolic compound. This is the first study to report the phytochemical screening, the content of phenolic compound, the antioxidant, anti-inflammatory and antiangiogenic activities of extract derived from Lophira procera. The use of this plant in traditional medicine against ulcers, breast cancer, kidney and dental pain is therefore justified and its potential as a candidate for bioactive therapeutic molecule.

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Background: The search for new anti-cancer molecules is one of the main concerns of oncology researchers. Scyphocephalium ochocoa is a plant of Myristicaceae family, used in traditional medicine against inflammatory diseases and several types of cancer. It is well established that free radicals, chronic inflammation and angiogenesis play an important role in initiation, tumor progression and metastasis formation. The aim of this study was to carry out a phytochemical screening, to determine the phenolic compounds content, to investigate the antiangiogenic, anti-inflammatory and antioxidant activities of water, water-ethanol and ethanol extracts of S. ochocoa. Methods: Phytochemical screening and determination of phenolic compounds content were performed using standard methods. Antiangiogenic activity was assessed using chick chorioallantoic membrane (CAM) model and Drabkin test. Anti-inflammatory activity was estimated by protein denaturation and erythrocyte membrane stabilization method. Finally the antioxidant activity was appreciated by DPPH radical inhibition and phosphomolybdenum assay. Results: The results of phytochemical studies show that extracts of bark of S. ochocoa are rich in polyphenols, tannins, flavonoids, proantocyanidins, saponosides, flavonols, flavanonols, sterol and triterpenes. The water extract showed good antiangiogenic activity (IC 50 = 1.153 μg/mL). Strong anti-inflammatory activity was observed with all extracts, IC 50 ranging from 34.775 ± 2.543 μg/mL to 74.577 ± 3.456 μg/mL for protein denaturation inhibition test and IC 50 values ranging from 36.793 ± 0.529 μg/mL at 48.912 ± 0.957 μg/mL for antihemolytic activity. In addition, all extracts showed good antioxidant activity marked by a strong inhibition of the DPPH radical (IC 50 ranging from 4.969 ± 0.263 μg/mL to 16.188 ± 0.336 μg/mL and AAI ranging from 3.090 ± 0.065 to 10.080 ± 0.517) and by greater total antioxidant capacity (with contents ranging from 37.654 ± 0.995 to 131.302 ± 1.102 VtCE (mg)/g dry extract).

The goal of this work is to evaluate the antioxidant anti-inflammatory properties, the phenolic compounds content, and antimicrobial potential of water–acetone, water–ethanol, and water extracts of Pachylobus balsamifera. The phenolic compounds content was evaluated to estimate their effect on the antioxidant, anti-inflammatory, and antimicrobial potential of the plant. Antioxidant activities were examined by DPPH (2,2-diphenyl-1- picrylhydrazyl) and ABTS (2,2′-azino-bis) assays. Antiinflammatory activity was determined by the proteins denaturation inhibition method. All plant extracts were evaluated against six reference strains, eleven clinical isolates, and two fungal strains. Phenols content was highest in the water–acetone and water–ethanol extracts. The water extract showed strong anti-inflammatory effect. The water–acetone extract presented a strong antioxidant property and the highest antimicrobial activities against Pseudomonas aeruginosa, Acinetobacter baumannii, Salmonella Sp., and Neisseria meningitidis. The tested microorganisms showed sensitivity to all extracts of the plant with the exception of Escherichia coli 105182 CIP, Listeria innocua LMG 135668 BHI, Salmonella enterica, Salmonella typhi, and Neisseria gonorrhea. Our results suggest that Pachylobus balsamifera extracts contain antioxidant, anti-inflammatory, and antimicrobial properties.

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