The study was conducted from November 2015 to November 2016 to determine bacterial load and identify pathogenic bacteria (S. aureus,E. coli, andSalmonellaespecies) in meat from abattoir and butcher shops as well as to assess associated hygienic and sanitation practices being experienced in the selected study site. A cross-sectional study was conducted where a simple random sampling method was used to select butcher shops, and the municipal abattoir was purposively selected. A structured questionnaire survey was also used to assess hygienic status of the municipal abattoir and butcher shops. A total of 124 samples (48 swab samples from abattoir carcass, 4 samples of carcass washing water about 20 ml of each, and 36 swab samples each from butcher shop cutting table and cutting knife, respectively) were collected during the study period. The collected samples were processed for aerobic plate count, and the total mean count was found to be 4.53 log10 cfu/cm2from abattoir carcass swab samples, 2.4 log10 cfu/ml from water samples, 6.58 log10 cfu/cm2from butcher shops cutting table, and 6.1 log10 cfu/cm2from cutting knife swab samples.E. coliwas the dominant bacterial species isolated (35.2%), followed byS. aureus(22.5%) andSalmonellaespecies (9.9%). According to the questionnaire survey, 48.4% (15/31) of the abattoir workers did not receive any training regarding food safety issues. Moreover, a majority (66.67%) of the respondents of the butcher house workers were grade 1–4 (elementary) in their educational level and do not use hairnet and handle money with bare hands during serving meat to consumers. The study showed that the hygienic status of the abattoir and butcher shops in the study area is poor, and the obtained results of bacterial load are higher than the acceptable limit of the standard. Therefore, the necessary strategies towards hygiene and sanitation of meat in the town should be implemented.

Background: Trypanosomosis is the most prevalent disease and causes serious economic losses in Ethiopia. The disease is among the major constraint to camel production in the country. Hence, this study aimed to determine the occurrence and associated risk factors of camel trypanosomosis in the Borana zone, Ethiopia. Methods: A cross-sectional study design was conducted from December 2018 to December 2019 using parasitological techniques in the Yabelo and Gomole districts of the Borana zone. A total of 384 camels were randomly selected and tested using wet blood film (thin) and the Giemsa-stained technique. The packed cell volume (PCV) of sampled camels was recorded using a hematocrit reader. Results: An overall 3.91% prevalence of camel trypanosomosis was observed in study areas. Trypanosoma-infected camel had significant (P=0.0001) lower mean PCV (20.73±1.91) than non-infected camel (27.07±4.35). The current study showed body condition of camel (OR=6.8, 95% CI: 1.44-31.83) and season (OR=3.1, 95% CI: 1.04-9.07) as risk factors for the occurrence of camel trypanosomosis. However, the sex, age, and origin (district) of camel did not affect the occurrence of camel trypanosomosis in the study areas. Conclusion: Camel trypanosomosis impeded camel production in the study areas. Therefore, control and prevent methods considering seasonality, different ecology is necessary to design and implement. Moreover, a further study involving more sensitive molecular techniques should be conducted on camel trypanosomosis and their vectors.

Reproduction is a basic requirement for efficient cattle production to continue or cease; whereas, reproductive disorders are the leading cause of economic loss in the cattle industry. To determine the prevalence and associated risk factors of major reproductive disorders in cattle in the Tole district, a cross-sectional study design was conducted from October, 2020, to October, 2021. To identify potential risk factors for cattle reproductive disorders, a multivariable random effect logistic regression analysis was used. A total of 400 randomly selected cows were examined, and 37.8% of them had at least one reproductive disorder. The three main reproductive disorders observed in the study area were abortion (17.8%), stillbirth (4.8%), and dystocia (3.3%). Risk factors for reproductive disorders in the study area included the calving season (OR = 3.7, 95% CL-1.37–7.18) and the accessibility of dogs to cattle (OR = 2.5, 95% CL-1.64–3.85). Accessibility of dogs to cattle in the study areas was also a risk factor for abortion (OR = 7.5, 95% CL-3.59–15.54). The present study also identified parity (OR = 5.2, 95% CL-1.05–26.04) as a risk factor for dystocia in cattle in the study areas. This demonstrated that losses in cattle production in the study areas are significantly attributed to reproductive problems. Hence, it is essential to increase public awareness of how reproductive disorders affect cattle production and to develop and implement appropriate control measures for these disorders. The causes of abortion and dystocia in various areas should be precisely identified and described through further research.

A cross-sectional study was conducted to isolate and identify of Escherchia coli, Pastuerella multocida, Salmonella gallinarum and Salmonella pullorum from the holding grounds of five purposively selected Addis Ababa live bird markets from November 2016 to May 2017 using bacterial culture, grams staining and biochemical testing. A total of 90 pooled fecal samples were collected as both deep (35) (bottom layer of the feces) and surface (55) (top layer of the feces). The specimens were inoculated directly into blood agar and incubated at 37°C for 24 h. After subsequent subcultures of the colony on the blood agar, to get pure colonies, the isolates were cultured on MacConkey agar and Salmonella shigella agar to differentiate bacterial colonies. Gram staining and biochemical tests were carried out on the pure colonies and a loop of the isolates were inoculated into nutrient broth and kept for further investigation of the bacteria. The results of the study revealed that, out of the 90 total samples examined, 32 (35.55%) of the samples were found to be positive for E. coli. Based on the sample types the study indicated that 55 (61.10%) surface fecal samples, 32 (58.20%) samples were positive for E. coli, and in 35 (38.90%) deep fecal layers, there was no E. coli positive samples. There was a statistically significant difference between sample types and E. coli isolation rate (p< 0.05). P. multocida, S. gallinarum and S. pullorum were not found during this study. Live bird markets may serve as source of public health and economically important bacteria; however, further microbiological and epidemiological investigation is needed to determine the types of major bacteria predominating in the Addis Ababa live-bird markets and the roles and magnitudes played by these markets in the epidemiology of these pathogens.

Trypanosomosis is a serious disease that causes a significant production loss in cattle. A cross sectional study was conducted in Jimma Horro District of Kellem Wollega Zone, Western Ethiopia to determine prevalence and associated risk factors of bovine trypanosomosis from October 2016 to October 2017. Blood samples from randomly selected 384 cattle of both sex and different age group were collected and examined with parasitological techniques. The overall prevalence of bovine trypanosomosis was 3.7% (14/384) in the study areas. The infection was highest due to Trypanosome congolense (50%) followed by Trypanosome vivax (28.6%) and Trypanosoma brucei (21.4%). Multivariable logistic regression analysis identified body condition as risk factors (P<0.05) for trypanosomosis in the district. However, there were no statistically significant difference observed among age groups, sex, skin color and different peasant associations (P> 0.05). The overall mean Packed Cell Volume (PCV) value was statistically significant difference between aparasitaemic and parasitaemic cattle (P< 0.05).The study showed that bovine trypanosomosis is one of the constraints to cattle production in Jimma Horro District. Hence, there is a need to create awareness about impact of disease on cattle production and appropriate control methods of trypanosomosis should be designed and implemented.