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The mammary gland is basically an exocrine gland, which basic function is to produce milk to feed the offspring. Mastitis is an inflammation with or without an infection of the mammary gland and a common problem in bitches of all breeds at various ages. The disease occurs primarily in non-spayed bitches during the postpartum period and more frequently, from 6 th to 10 th day after whelping. A two years old mongrel bitch was presented in Teaching Veterinary Clinical Complex, College of Veterinary Sciences and Animal Husbandry, Selesih with a history of anorexia, vomition and sudden swelling of mammary glands. The case was diagnosed as mastitis based on history, clinical signs and bacteriological examination. Bacterial culture showed presence of Staphylococcus aureus as a causative agent and then subjected to commonly used antimicrobials to determine their sensitivity. The bitch was successfully treated with a course of parenteral antibiotic and supportive therapy along with proper care and management till clinical recovery.

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