This study has investigated the impact of gender inequality and environmental degradation on human well-being in the case of Pakistan from 1980 to 2019. Augmented dickey-fuller unit root test is used for stationarity of the variables. The autoregressive distributed lag model (ARDL) is used for co-integration among the variables of the model. The results show that gender inequality has a negative and significant impact on human well-being in Pakistan, while gender equality encourages human well-being. The calculated results show that there is a positive, but insignificant relationship between environmental degradation and human well-being in the case of Pakistan. The estimated results show that economic misery has a negative and significant impact on human well-being in the case of Pakistan. The estimated results show that economic growth has a positive and significant relationship with human well-being in Pakistan. Based on estimated results, it is concluded that gender equality, economic misery, and economic growth are playing an important role in determining human well-being in Pakistan. Therefore, to improve human well-being, the government must reduce gender inequality and economic misery while enhancing in parallel economic growth.