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In-depth and comprehensive knowledge on the immune cell activities and their correlation with severity of sepsis will help clinicians and scientists to design effective immunomodulatory therapeutics for treating sepsis.

The objective of this study is to synthesize and characterize collagen grafted poly(3-hydroxylbutyrate-co-3-hydroxylvalerate) (PHBV) film for loading of BSA capped silver (Ag/BSA) nanoparticles. Thermal radical copolymerization and aminolysis methods were used to functionalize macroporous PHBV, followed by collagen grafting so as to formulate collagen-g-poly(hydroxyethylmethyl acrylate)-g-poly(3-hydroxylbutyrate-co-3-hydroxylvalerate) [collagen-g-PHEMA-g-PHBV] and collagen-g-aminated-poly(3-hydroxylbutyrate-co-3-hydroxylvalerate) [collagen-g-NH2-PHBV] films, respectively. Spectroscopic (FTIR, XPS), physical (SEM), and thermal (TGA) techniques were used to characterize the functionalized PHBV films. The amount of collagen present on grafted PHBV film was quantified by the Bradford method. The Ag/BSA nanoparticles were then loaded on collagen grafted and untreated PHBV films, and the nanoparticles loading were determined by atomic absorption spectrometry. The amount of nanoparticles loaded on collagen grafted PHBV film was found to be significantly greater than that on the untreated PHBV film. The nanoparticles loaded PHBV film can potentially serve as a scaffold to promote the growth of bone cells while inhibiting the bacterial growth.

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There has been a growing interest in the use of protein conjugated nanoparticles for applications in biomedical, sensing, and advanced imaging. The objective of this study was to understand the interaction of protein conjugated silver nanoparticles (Ag/BSA NPs) with biological substrate (collagen layer). The adsorption behavior of synthesized Ag/BSA NPs on collagen immobilized silanized surface was followed by UV-vis spectroscopy by initially studying the formation of collagen layer and subsequent adsorption of Ag/BSA NPs to the immobilized layer. Surface plasmon resonance (SPR) data provided the real time profile of adsorption of Ag/BSA NPs from solution onto collagen immobilized and control substrates as well as desorption of nanoparticles from the substrates. The retention of NPs to substrate is sensitive to chemistry of the underlying substrate and on the external environment. UV-vis and atomic absorption spectrometric analysis of Ag/BSA NPs desorption performed under different pH conditions showed more NPs retained at physiological pH than the acidic and basic conditions. Nanoparticles retention on collagen immobilized substrate at physiological pH could influence properties of biological interest such as circulation lifetime and biodistribution of nanoparticles in the body.