Synthesis and Characterization of Collagen Grafted Poly(hydroxybutyrate–valerate) (PHBV) Scaffold for Loading of Bovine Serum Albumin Capped Silver (Ag/BSA) Nanoparticles in the Potential Use of Tissue Engineering Application

Abstract

The objective of this study is to synthesize and characterize collagen grafted poly(3-hydroxylbutyrate-co-3-hydroxylvalerate) (PHBV) film for loading of BSA capped silver (Ag/BSA) nanoparticles. Thermal radical copolymerization and aminolysis methods were used to functionalize macroporous PHBV, followed by collagen grafting so as to formulate collagen-g-poly(hydroxyethylmethyl acrylate)-g-poly(3-hydroxylbutyrate-co-3-hydroxylvalerate) [collagen-g-PHEMA-g-PHBV] and collagen-g-aminated-poly(3-hydroxylbutyrate-co-3-hydroxylvalerate) [collagen-g-NH2-PHBV] films, respectively. Spectroscopic (FTIR, XPS), physical (SEM), and thermal (TGA) techniques were used to characterize the functionalized PHBV films. The amount of collagen present on grafted PHBV film was quantified by the Bradford method. The Ag/BSA nanoparticles were then loaded on collagen grafted and untreated PHBV films, and the nanoparticles loading were determined by atomic absorption spectrometry. The amount of nanoparticles loaded on collagen grafted PHBV film was found to be significantly greater than that on the untreated PHBV film. The nanoparticles loaded PHBV film can potentially serve as a scaffold to promote the growth of bone cells while inhibiting the bacterial growth.

Formulation of silver chloride/poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (AgCl/PHBV) films for potential use in bone tissue engineering

“…Pure PHBV film served as a control for the measurement. The thermograms of blend films (before and after partial washing) and PHBV film showed a one‐step degradation between 290 and 300 °C (Figure ) . Several researchers have reported a change in thermal profile of PHBV upon chemically or physical modification which is in agreement with the DTG results reported, i.e., there was a significant difference in the recorded char yield of the films at 400 °C.…”

Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

“…As it is shown, the high lipase loading was observed for nanoparticles coated with the mixture of chitosan and collagen crosslinked traditionally with Glu. The crosslinked collagen coated on the surfaces of nanoparticles did not distort the Bradford test quality-collagen needs to be treated with 6 M HCl under reflux to be detectable in this method [32]. In all cases, crosslinking the polymeric shell with squaric acid (SqA) reduced the amount of immobilized lipase on the support surface.…”

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

“…Bovine serum albumin capped Ag nanoparticles have proved to be biocompatible and maintained stability over a wide range without sacrificing their antibacterial activity remarkably. Once loaded into the collagen modified PHBV film via simple immersion method, the bovine serum albumin produced strong electrostatic interaction with collagen so as to bind the nanoparticles on the grafted PHBV film . Ag‐loaded PHBV can potentially serve as a scaffold to promote the growth of bone cells and inhibit the bacterial growth as well.…”