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Anaerobic butanol producing bacteria were isolated, among them Clostridium sporogens found to be a potent producer of butanol with the production of 0.1 g/L of butanol. Individual fermentation parameters including inoculum age (10 -24 hrs), size (1 % -9 % v/v) were optimized and maximum production was observed on 12 hrs old 8 % (v/v) inoculum. From the single parameter optimization results, for further improvement in the production of butanol, media engineering was done using Plackett-Burman (PB) and Central Composite Design (CCD). Production of butanol increased to 1.5 g/L and the effect of different media components in butanol production is evaluated.