Simultaneous saccharification and fermentation of oil palm front for the production of 2,3-butanediol

Hazeena, Sulfath Hakkim; Salini, Chandrasekharan Nair; Sindhu, Raveendran; Pandey, Ashok

doi:10.1016/j.biortech.2019.01.042

Cited by 47 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, Cheng et al [49] employed a K. pneumoniae strain M5al in batch cultivation at a bioreactor level and achieved 18 g/L PDO and up to 5 g/L of BDO after 18 h of cultivation, while the pH was maintained at 6.8 through the automatic addition of NaOH, and the starting substrate concentration was 40 g/L of glycerol. Da PDO is a three-carbon diol with an important contribution in ecological materials division (e.g., biopolymers, polyesters, composites, coatings), while BDO is a four-carbon diol with a major role in the industry of polymers [34,35,48]. Considering the results obtained for PDO and BDO (Table 1, Figure 1), these were similar to those reported in the literature.…”

Section: Resultssupporting

confidence: 87%

“…A higher pH tolerance (e.g., 8) was related to the genetically modified strains, which were optimized for an elevated metabolite production such as PDO [47], while a lower pH level inhibits cell division [46]. PDO is a three-carbon diol with an important contribution in ecological materials division (e.g., biopolymers, polyesters, composites, coatings), while BDO is a four-carbon diol with a major role in the industry of polymers [34,35,48]. Considering the results obtained for PDO and BDO (Table 1, Figure 1), these were similar to those reported in the literature.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Mitrea

Vodnar

2019

Pathogens

View full text Add to dashboard Cite

Despite being a well-known human pathogen, Klebsiella pneumoniae plays a significant role in the biotechnology field, being considered as a microbial cell factory in terms of valuable chemical biosynthesis. In this work, Klebsiella pneumoniae DSMZ 2026 was investigated for its potential to biosynthesize 1,3-propanediol (PDO) and 2,3-butanediol (BDO) during batch fermentation under controlled and uncontrolled pH levels. The bacterial strain was cultivated at a bioreactor level, and it was inoculated in 2 L of specific mineral broth containing 50 g/L of glycerol as the main carbon source. The process was conducted under anaerobic conditions at 37 • C and 180 RPM (rotations per minute) for 24 h. The effect of pH oscillation on the biosynthesis of PDO and BDO was investigated. Samples were taken every 3 h and specific tests were performed: pH measurement, main substrate consumption, PDO and BDO production. The cell morphology was analyzed on both solid and liquid media. After 24 h of cultivation, the maximum concentrations of PDO and BDO were 28.63 ± 2.20 g/L and 18.10 ± 1.10 g/L when the pH value was maintained at 7. Decreased concentrations of PDO and BDO were achieved (11.08 ± 0.14 g/L and 7.35 ± 0.00 g/L, respectively) when the pH level was not maintained at constant values. Moreover, it was identified the presence of other metabolites (lactic, citric, and succinic acids) in the cultivation media at the beginning of the process, after 12 h and 24 h of cultivation.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Mitrea

Vodnar

2019

Pathogens

View full text Add to dashboard Cite

show abstract

“…Residual saccharin sugars, lignocellulosic materials, and the biodiesel‐derived glycerol were the most used feedstocks in bio‐based 2,3‐BDO production. The main raw materials verified were: artichoke biomass, 37,55–57 vinegar, 36 corn hydrolysate, 58,59 xylose, 6,33,60–63 whey, 31,43 oil palm, 64 cassava, 35,44 and crude glycerol 50,65 . Despite presenting a reduction in the long term, research on the substrate must still be extensively carried out due to its importance in the bioprocess economy on a large scale, as substrate costs can represent up to 50% of the production costs 11 .…”

Section: Resultsmentioning

confidence: 99%

Technological development of the bio‐based 2,3‐butanediol process

Tinôco

Borschiver

Coutinho

et al. 2020

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

The biotechnological production of 2,3‐butanediol (2,3‐BDO) has economic and environmental advantages over the chemical route, although its large‐scale implementation has not yet been consolidated. To establish the technological maturity of the bio‐based 2,3‐BDO process, and the factors limiting its industrial use, a technology roadmap was built from patents and scientific articles, in which four drivers were identified: production process, recovery process, product, and application. The production process driver was reported in 53.25% of documents, notably including studies on substrates, engineered microorganisms, efficient production methods, and nutritional supplementation. The recovery process driver was investigated in 23.5% of studies, with conventional distillation and solvent extraction being the main technologies performed on a large scale. Product and application drivers accounted for 9.25% and 14% of studies, respectively, highlighting the chemical intermediates, plastics, food additives, and cosmetics market segments. A limited number of companies like LanzaTech and GS Caltex Corporation already produce industrially bio‐based 2,3‐BDO, while academic centers such as the Yancheng Institute of Technology are focused to the laboratory scale with technologies to be implemented in the long term. This study can therefore help to establish and gain an understanding of the necessary strategies to consolidate the industrial production of bio‐based 2,3‐BDO in the future. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

“…The effect of enzyme loading on batch cellulose hydrolysis was studied at the optimal condition of temperature 50 °C and pH 4.5 [ 34 – 36 ]. It is evident from Fig.…”

Section: Resultsmentioning

confidence: 99%

Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation

Prasad

Sahoo

Naveen

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Simultaneous saccharification and fermentation (SSF) of pre-treated lignocellulosics to biofuels and other platform chemicals has long been a promising alternative to separate hydrolysis and fermentation processes. However, the disparity between the optimum conditions (temperature, pH) for fermentation and enzyme hydrolysis leads to execution of the SSF process at sub-optimal conditions, which can affect the rate of hydrolysis and cellulose conversion. The fermentation conditions could be synchronized with hydrolysis optima by carrying out the SSF at a higher temperature, but this would require a thermo-tolerant organism. Economically viable production of platform chemicals from lignocellulosic biomass (LCB) has long been stymied because of the significantly higher cost of hydrolytic enzymes. The major objective of this work is to develop an SSF strategy for D-lactic acid (D-LA) production by a thermo-tolerant organism, in which the enzyme loading could significantly be reduced without compromising on the overall conversion. Results A thermo-tolerant strain of Lactobacillus bulgaricus was developed by adaptive laboratory evolution (ALE) which enabled the SSF to be performed at 45 °C with reduced enzyme usage. Despite the reduction of enzyme loading from 15 Filter Paper Unit/gLCB (FPU/gLCB) to 5 FPU/gLCB, we could still achieve ~ 8% higher cellulose to D-LA conversion in batch SSF, in comparison to the conversion by separate enzymatic hydrolysis and fermentation processes at 45 °C and pH 5.5. Extending the batch SSF to SSF with pulse-feeding of 5% pre-treated biomass and 5 FPU/gLCB, at 12-h intervals (36th–96th h), resulted in a titer of 108 g/L D-LA and 60% conversion of cellulose to D-LA. This is one among the highest reported D-LA titers achieved from LCB. Conclusions We have demonstrated that the SSF strategy, in conjunction with evolutionary engineering, could drastically reduce enzyme requirement and be the way forward for economical production of platform chemicals from lignocellulosics. We have shown that fed-batch SSF processes, designed with multiple pulse-feedings of the pre-treated biomass and enzyme, can be an effective way of enhancing the product concentrations.

show abstract

Simultaneous saccharification and fermentation of oil palm front for the production of 2,3-butanediol

Cited by 47 publications

References 31 publications

Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Technological development of the bio‐based 2,3‐butanediol process

Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation

Contact Info

Product

Resources

About