This work is devoted to the evaluation of the efficiency of a new low-temperature plasma jet driven in ambient air by a dc-corona discharge to inactivate adherent cells and biofilms of Gram-positive bacteria. The selected microorganisms were lactic acid bacteria, a Weissella confusa strain which has the particularity to excrete a polysaccharide polymer (dextran) when sucrose is present. Both adherent cells and biofilms were treated with the low-temperature plasma jet for different exposure times. The antimicrobial efficiency of the plasma was tested against adherent cells and 48 h-old biofilms grown with or without sucrose. Bacterial survival was estimated using both colony-forming unit counts and fluorescence-based assays for bacterial cell viability. The experiments show the ability of the low-temperature plasma jet at atmospheric pressure to inactivate the bacteria. An increased resistance of bacteria embedded within biofilms is clearly observed. The resistance is also significantly higher with biofilm in the presence of sucrose, which indicates that dextran could play a protective role.

No abstract

Aircrafts require a large amount of fuel in order to generate enough power to perform a flight. That consumption causes the emission of polluting particles such as carbon dioxide, which is implicated in global warming. This paper proposes an algorithm which can provide the 3D reference trajectory that minimizes the flight costs and the fuel consumption. The proposed algorithm was conceived using the Floyd–Warshall methodology as a reference. Weather was taken into account by using forecasts provided by Weather Canada. The search space was modeled as a directional weighted graph. Fuel burn was computed using the Base of Aircraft DAta (BADA) model developed by Eurocontrol. The trajectories delivered by the developed algorithm were compared to long-haul flight plans computed by a European airliner and to as-flown trajectories obtained from Flightradar24®. The results reveal that up to 2000 kg of fuel can be reduced per flight, and flight time can be also reduced by up to 11 min.