We presented a green and simple method to synthesize carbon nanodots (C-dots) from millets using hydrothermal synthesis route for the first time. The obtained C-dots have average diameter ranging from 6 to 10 nm. Optical measurements showed the insight into the formation of functional groups on the particle surfaces, resulting in their good water solubility and bioconjugation. After treatment with C-dots, small subpopulation of the human cervical tumor cells became bright and exhibited multicolor fluorescence under different excitation wavelength. The achievement demonstrated potential applications of fluorescent C-dots in the field of biomedical application.

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We presented a low-cost and simple method to synthesize carbon nanodots (CDs) from waste wine cork using hydrothermal synthesis.  The structural and optical properties of the CDs are characterized by TEM, FTIR, Raman, UV-Vis absorption, and photoluminescence (PL) spectra. The analysis results indicated the average diameter of CDs ⁓ 6.2 ± 2.7 nm. Optical measurements showed the phenomenon of excitation-dependent PL and the formation of functional groups on the surface of the particles. CDs with a quantum yield of 1.54% was calculated using quinine sulfate as reference. Furthermore, a probe of wine cork-derived CDs in bioimaging has been successfully applied in living mesenchymal stem cells (MSCs). After treatment with CDs, MSCs exhibited fluorescence including green, yellow, and red colors under the excitation wavelengths in the range 330–385 nm, 450–480 nm, and 510–550 nm, respectively. The achievement demonstrated potential applications of fluorescent CDs in the field of the fluorescent image.

Traumatic brain injury (TBI) is a complicated topic.TBI has expensive treatment, sequelae, high mortality and efficient therapeutic strategies for TBI are urgently needed. Patients with severe TBI account for 10% of all traumatic brain injury patients, but more than 80% of patients who die from traumatic brain injury are in this group of patients. That proves the danger of severe TBI. Mesenchymal stem cells (MSCs) are multipotent cells with high proliferative and self-renewal capacities, as well as immunomodulatory and neuroregenerative effects. MSCshave been shown to treat TBI and significantly reduce inflammation of injured tissue. In the present study, we evaluated the quality and effectiveness of mesenchymal stem cell transplantation with the graft dose of 9x106cells/kg. Clinical indexes (Pulse, temperature, SBP, HATTr, HATTB, GCS) and subclinical (Hepatic index, kidney, cortisol, lactate, glucose, hematological index, respiratory index) were monitored on 60 patients within 6 months. The results showed that the recovery journey is different for every person with the graft dose of 6-9x106 cells/kg, but there were no adverse complications for the patients, the clinical and paraclinical indicators were stable. No significant side effects were observed during the trial as well as after 6 months of treatment. The MSC transfusions significantly increased the life quality patients; reduced brain damage  and increased HSC counts. Inflammatory cytokines (IL-6) levels decreased and GCS increased in the MSC transfusion group, indicating that the patient's health improved.

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