Acoustic micro-beams produced by highly focused ultrasound transducer have been investigated for micro-particle and cell manipulation. Here we report the selective trapping of microspheres via the acoustic force using the single acoustical beam. The forbidden band theory of acoustic radiation force trapping is proposed, which indicates that the trapping of particles via the acoustic beam is directly related to the particle diameter-to-beam wavelength ratio as well as excitation frequency of the ultrasonic acoustic tweezers. Three tightly focused LiNbO 3 transducers with different center frequencies were fabricated for use as selective single beam acoustic tweezers (SBATs). These SBATs were capable of selectively manipulating microspheres of sizes 5-45 mm by adjusting the wavelength of acoustic beam. Our observations could introduce new avenues for research in biology and biophysics by promoting the development of a tool for selectively manipulating microspheres or cells of certain selected sizes, by carefully setting the acoustic beam shape and wavelength.Recently, single beam acoustic tweezers (SBATs), analogous to optical tweezers for trapping and manipulating the individual particle with an acoustic micro-beam (

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The manipulation of acoustic waves plays an important role in a wide range of applications. Currently, acoustic wave manipulation typically relies on either acoustic metasurfaces or phased array transducers. The elements of traditional metasurfaces are usually designed with complex artificial structures and limited by the additive manufacturing capability, which are difficult to apply in MHz frequency underwater acoustic wave control. Phased array transducers, suffering from high-cost and complex control circuits, are usually limited by the array size and the filling ratio of the control units. Coding piezoelectric metasurfaces are reported; three wave functionalities, including beam steering, beam focusing, and vortex beam focusing are demonstrated; and acoustic tweezers and ultrasound imaging are eventually realized. The information coded on the piezoelectric metasurfaces herein is frequency independent and originates from the polarization directions, pointing either up or down, of the piezoelectric materials. Such a piezoelectric metasurface is driven by a single electrode and acts as a controllable active sound source, which combines the advantages of acoustic metasurfaces and phased array transducers while keeping the devices structurally simple and compact. The coding piezoelectric metasurfaces lead to potential technological innovations in underwater acoustic wave modulation, acoustic tweezers, biomedical imaging, industrial nondestructive testing, and neural regulation.

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