No abstract

Deep eutectic solvents (DESs) are a mixture of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) molecules that can consist, respectively, of natural plant metabolites such as sugars, carboxylic acids, amino acids, and ionic molecules, which are for the vast majority ammonium salts. Media such as DESs are modular tools of sustainability that can be pointed toward the extraction of bioactive molecules due to their excellent physicochemical properties, their relatively low price, and accessibility. The present review focuses on the application of DESs for protein extraction and purification. The in-depth effects and principles that apply to DES-mediated extraction using various renewable biomasses will be discussed as well. One of the most important observations being made is that DESs have a clear ability to maintain the biological and/or functional activity of the extracted proteins, as well as increase their stability compared to traditional solvents. They demonstrate true potential for a reproducible but more importantly, scalable protein extraction and purification compared to traditional methods while enabling waste valorization in some particular cases.

Similarity search is a core component in various applications such as image matching, product recommendation and low-shot classification. However, single machine solutions are usually insufficient due to the large cardinality of modern datasets and stringent latency requirement of on-line query processing. We present Pyramid, a general and efficient framework for distributed similarity search. Pyramid supports search with popular similarity functions including Euclidean distance, angular distance and inner product. Different from existing distributed solutions that are based on KD-tree or locality sensitive hashing (LSH), Pyramid is based on Hierarchical Navigable Small World graph (HNSW), which is the state of the art similarity search algorithm on a single machine. To achieve high query processing throughput, Pyramid partitions a dataset into sub-datasets containing similar items for index building and assigns a query to only some of the sub-datasets for query processing. To provide the robustness required by production deployment, Pyramid also supports failure recovery and straggler mitigation. Pyramid offers a set of concise API such that users can easily use Pyramid without knowing the details of distributed execution. Experiments on large-scale datasets show that Pyramid produces quality results for similarity search, achieves high query processing throughput and is robust under node failure and straggler.

Optical sectioning with high-throughput, a high signal-to-noise ratio (SNR), and submicrometer resolution is crucial, but challenging, to three-dimensional visualization of large biological tissue samples. Here we propose line-scanning imaging with digital structured modulation for optical sectioning. Our method generates images with a significantly improved SNR, compared to wide-field structured illumination microscopy (WF-SIM), without residual modulation artifacts. We image a 14.5 m m × 11.5 m m horizontal view of mouse brain tissue at a pixel resolution of 0.32 µ m × 0.32 µ m in 101 s, which, compared to WF-SIM, represents a significant improvement on imaging throughput. These results provide development opportunities for high-throughput, high-resolution large-area optical imaging methods.