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This work evaluated the antioxidative properties of ethyl acetate extracts from Aspergillus candidus CCRC 31543 broth filtrate (EAEAC). EAEAC markedly exhibited antioxidative activity in a soybean oil peroxidation system as measured by the Rancimat method. This finding significantly correlated (r=0·9702, P<0·05) with the extent of antioxidative activity in a linoleic acid peroxidation system as measured by the thiocyanate method. EAEAC also displayed good thermal stability when heated at 185°C for 2 h. Although the antioxidative activity of the EAEAC was unstable at the alkali pH value, it was more effective at the neutral and acidic pH values. EAEAC had synergistic effects with α‐tocopherol and citric acid in linoleic acid peroxidation system. EAEAC showed no mutagenicity to Salmonella typhimurium TA98 and TA100 as evaluated by Ames test. These properties indicated that Aspergillus candidus CCRC 31543 might be a potential antioxidant for application in food products. ©1997 SCI

Microvascular free flap surgery has been a reliable reconstruction method, but some flaps may have circulatory compromise within five to seven days of surgery. Besides, many auxiliary devices are expensive and complex to operate. Therefore, most of the medical personnel still use the manual observation. To decrease heavy workload on medical personnel, the monitoring system was developed to analyze the color variation of free flap by a visible-light camera with advantages such as fast operation, low cost, noninvasive behavior, and contactless and nonradiative operation would be also needed for patients. The proposed system was mainly comprised of a longitudinal image registration without markers and a circulatory compromise detection, which were aiming to reduce the deformation from patients' motion and eliminate the influence of illumination variation from the surrounding, respectively. In the clinical trial, the performance assessment of image registration reveals that the average and the standard variation of dice similarity coefficient (DSC) can reach 0.959 and 0.011, respectively. The relationship among the situation of blood perfusion and the results of circulatory compromise detection at flap was summarized and shown that the detection system not only determined the moment of vascular occlusion but also the type of occlusion. In addition, vascular congestion was even detected much earlier than manual observation. Therefore, our monitoring system is expected to relieve the workload on medical personnel and could be a reliable tool for the surgeon to hold onto the chance of repairing the free flap with vascular obstruction.