Abstract:The purpose of this study was to investigate the its helical integrity. This made penetration of enzymes into in vitro degradation potential of porcine pericardia fixed with biological tissue easier. Of the multifunctional EC test groups, various aldehyde or epoxy compound (EC) fixatives, using tissues fixed with tetrafunctional EC (EX-521) or trifunctional bacterial collagenase and pronase. The fixatives investigated EC (EX-313) had relatively better resistance to degradation were formaldehyde (FA), glutaraldehyde (GA), monofuncthan those fixed with bifunctional EC (EX-810). The extent tional EC (EX-131), and multifunctional ECs (EX-810, EXof degradation for the EX-313 or EX-512 EC fixed tissues was 313, and EX-512). Fresh porcine pericardium was used as a similar to that observed for the FA-or GA-fixed tissues. The control. The test samples were well immersed in a 20-