Starch synthesis in leaves was increased by phosphate starvation or by treatments which decreased cytoplasmic orthophosphate levels (such as mannose feeding). Usally less than 30% of the total carbon fixed during CO2 assimlation was incorporated into starch in spinach (Spinacia oleracca L.), spinach beet (Beta vulgaris), and tobacco (Nicotiana tabacum) leaves.In isolated spinach chloroplasts, formation of starch from CO2 was usualy less than in leaves.

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