Aims: Aim of the study is to identify accurately Stenotrophomonas maltophilia isolates recovered from environmental and clinical samples.
Methods and Results: Recovery of Sten. maltophilia‐like isolates from soil samples using the vancomycin, imipenem, amphotericin B (VIA) selective agar medium enabled distinction of various morphotype colonies. A set of soil and clinical isolates was tested for species identification using different methods. 16S rDNA analyses showed the dark green with a blue halo morphotype to be typical Sten. maltophilia strains. The API‐20NE, Vitek‐2 and Biolog phenotypic analyses typically used for the identification of clinical isolates did not perform well on these soil isolates. The species‐specific PCR screening targeting Sten. maltophilia 23S rDNA and the multiplex smeD/ggpS PCR, differentiating Sten. maltophilia from Stenotrophomonas rhizophila, were tested for improvement of these identification schemes. The latter multiplex PCR identified all isolates tested in this study, whatever be their origin.
Conclusions: Isolation on VIA medium and confirmation of Sten. maltophilia species membership by smeD PCR is proposed to identify environmental and clinical isolates of Sten. maltophilia.
Significance and Impact of the Study: The proposed approach enables isolation and identification of Sten. maltophilia from different environments in an easy and rapid way. This approach will be useful to accurately manage studies on the abundance and distribution of Sten. maltophilia in hospital and nonhospital environments.
This work aims at explaining and predicting the influence of dye doping and space charge effects on charge carrier transport at different operating temperatures. For that purpose, currentvoltage J-V characteristics for typical electrically-doped multilayer OLEDs have been simulated. The results are in good agreement with experiment.
Abstract— This work aims at explaining and predicting the influence of the thickness of organic materials, dye doping, and space‐charge effects on charge‐carrier transport at different operating temperatures for high current densities (50 ≤ J ≤ 7000 mA/cm2). For the purpose of determining these influences, current‐voltage characteristics for typical electrically doped multilayer organic light‐emitting diodes (OLEDs) have been simulated. The results of the simulations concur with experimental data.
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