This paper aimed to investigate the characteristics of female HPV infection in the Shangcheng District, Hangzhou city, China. The retrospective study was designed to analyze the HPV prevalence rate of 22,382 women receiving physical examinations from 2016 to 2020 in the Shangcheng District of Hangzhou city in China. A commercial kit was designed to detect the HPV genotypes. Trends were examined for age-specific groups (≤ 30 years, 31–44 years, 45–54 years, 55–64 years,  ≥ 65 years). A receiver operating characteristic (ROC) analysis was used to assess the correlation of age classification in high risk HPV (HR-HPV) infection. 22.41% (5015/22,382) of samples were HPV positive, 91.28% (4578/5015) of HPV positive women were infected by HR-HPV. The most prevalent HR-HPV genotypes were 16, 52, 18, 58, 56, and 51. The trend of HPV prevalence showed the significant differences in age-specific groups (χ2 = 164.70, P < 0.001). Moreover, the areas under ROC curve (AUC) was 0.712 in 55–64 years group which showed a strong contribution of age classification for HR-HPV infection. This study provided baseline data on the prevalence characteristics of HPV infection and the critical age group of HR-HPV prevalence rate was 55–64 y among the samples receiving physical examinations.

Purpose: To develop a dose constraint template to increase conformity index (CI) of the planning target volume (PTV) and improve intensity‐modulated radiotherapy (IMRT) planning efficiency for patients diagnosed with early stage nasopharyngeal carcinoma (NPC). Methods and Materials: Twenty patients who were diagnosed with early stage NPC under IMRT treatment were selected in this study. The target volumes for all twenty patients were delineated on CORVUS Treatment Planning System (TPS) by oncologists. We started IMRT planning for the first 10 patients. Two dose limiting regions (Dlrs) around PTV were added by extending 1 cm region from PTV in each direction. All the IMRT plans were created and approved according to the dose constraints criteria in RTOG 0225 protocol. The mean values of end points of the extended normal structures from all 10 patients were chosen as the planning dose constraint template. For the other 10 patients, the IMRT treatment plans were performed either with (Plan1) or without dose constraint template (Plan0) derived from the first group of patients. The does distribution and CI for PTV were compared for plan 1 and plan 0, as well as planning optimization time. Results: The clinical target doses from plan 1 were covered well and met the clinical criteria. The doses for normal critical structures of Plan 1 were comparable to Plan0. The CI of Plan1 was increased by 0.08±0.07 and the plan optimization time was decreased significantly. The average planning time for one NPC case was reduced to about 20 minutes excluding contouring time. Conclusions: The usage of dose constraint template together with Dlrs increases the CI of PTV and improves IMRT planning efficiency without sacrificing of target dose coverage and critical structure sparing for patients diagnosed with early stage NPC.