The purpose of this study was to develop a method for lidocaine detection in dental pulp by high-performance liquid chromatography. The amounts of lidocaine in dog pulps were quantitated after local injection to evaluate lidocaine recovery from pulp tissue with this technique. Comparison was also made between the amount of lidocaine found in upper and lower canines. The high-performance liquid chromatography system was shown to be a reliable and reproducible tool for lidocaine determination. Lidocaine extraction from the tissue showed recovery of 90%. The amount of lidocaine recovered from the upper canine (0.21 microg/mg) was higher than the lower canine (0.17 microg/mg).

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The sealing ability of various retrofilling materials was compared. The root canals of 85 single-rooted teeth were cleansed and obturated with gutta-percha without sealer using lateral condensation. The apical 3 mm of the roots were resected and divided into positive control, negative control, and five experimental groups. The experimental teeth received root-end cavity preparation to 3 mm depth using an ultrasonic retroprep tip. The retrocavities were dried and divided into five groups to receive the following materials: amalgam with varnish, amalgam with Clearfil Liner Bond II, thermoplasticized gutta-percha (TGP) with sealer, Ketac-fil, and Super-EBA. After immersion in India ink for 7 days, the roots were demineralized, cleared, and evaluated for dye leakage under a stereomicroscope. Statistical analysis showed that Super-EBA, Ketac-fil, and TGP with sealer demonstrated less leakage than amalgam with varnish and amalgam with Clearfil Liner Bond II (p < 0.05). Super-EBA also leaked significantly less than Ketac-fil or TGP sealer (p < 0.05). No significant difference was found between Ketac-fil and TGP or between the two groups filled with amalgam (p > 0.05).

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