A seven-year-old female spayed Schnauzer was presented with cutaneous ulcerated nodular lesions shortly after the beginning of an immunosuppressive treatment for immune-mediated hemolytic disease. Cytology was performed and a great number of neutrophils and banana-shaped organisms were observed. Biopsy showed a neutrophilic and histiocytic dermatitis and panniculitis with myriads of intralesional bradyzoites cysts and tachyzoites. PCR analysis was positive for Toxoplasma gondii and negative for Neospora caninum. Immunohistochemistry confirmed intralesional T. gondii antigens. This study reports a rare case of cutaneous toxoplasmosis in an immunosuppressed dog.
Keywords: dog, toxoplasmosis, cutaneous, immunosuppression
RESUMO
Uma cadela Schnauzer, castrada, de sete anos apresentou lesões cutâneas nodulares ulceradas pouco tempo após início de tratamento imunossupressor para doença hemolítica imunomediada. Foi realizado exame citológico, e um grande número de neutrófilos e estruturas em forma de banana foi observado.Biópsia mostrou dermatite e paniculite neutrofílica e histiocítica com miríades de taquizoítos e cistos de bradizoítos intralesionais. PCR foi positivo para Toxoplasma gondii e negativo para Neospora caninum. Imuno-histoquímica confirmou antígenos de T. gondii intralesionais. Este trabalho relata um caso raro de toxoplasmose cutânea em um cão imunossuprimido. Palavras-chave: cão, toxoplasmose cutânea, imunossupressão
The aim of the present study was to compare two protocols of Agar Gel Immunodiffusion (AGID) that can be used to diagnose Equine Infectious Anemia (EIA). Different volumes of reagents and support for agar gel were used, as recommended by the Ministry of Agriculture, Livestock and Food Supply (MAPA) and the World Organization for Animal Health (OIE). Equidae sera samples (n = 1000) were tested using a national commercial kit and different volumes (i.e. 25 µL-MAPA and 50 µL-OIE) of reagents and standard sera. Antigen was used as recommended by MAPA. The results were assessed by two operators, 48 hours after beginning the test. At the end of the experiment, a Kappa index of 1 was obtained by the operators, for the interpretation of the results and for the different volumes of reagents used. Similar results of agreement (Kappa index = 1) were obtained when using slides or Petri dishes as support for the agar gel. In both cases, an almost perfect agreement was achieved, suggesting that the use of different supports does not affect the results. These findings indicate that both protocols (MAPA and OIE) can be used to diagnose EIA.
Objetivou-se com este estudo determinar a ocorrência de anticorpos anti-BVDV e anti-BoHV-1 em búfalos no estado do Rio Grande do Norte. Foram analisadas 241 amostras séricas de oito machos e 233 fêmeas em idade reprodutiva por meio da técnica de vírus neutralização. Das amostras analisadas observou-se uma ocorrência de 76,7% de anticorpos anti-BVDV e 49,8% de anticorpos anti-BoHV-1. Entre as fêmeas positivas para ambos os agentes, havia animais com histórico de abortamento, destacando-se a associação da manifestação desse sinal clínico com a ocorrência de animais soropositivos. Foi evidenciada também a presença de machos positivos, o que contribui para a manutenção dos agentes no rebanho. Conclui-se que a elevada ocorrência de anticorpos anti-BVDV e anti-BoHV-1, podem ocasionar problemas reprodutivos na população bubalina estudada e que medidas de controle devem ser adotadas com o intuito de reduzir as perdas econômicas.
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