RESUMOA Bioinformática é entendida como uma área interdisciplinar do conhecimento, cujo objetivo é empregar técnicas computacionais, físicas e matemáticas para avaliar, gerar e gerenciar informações biológicas. Desse modo, este trabalho tem por objetivo incentivar o uso contínuo de softwares de Bioinformática durante a disciplina de Bioquímica na modalidade a distância por meio do uso de um guia sumarizado, o qual poderá ser empregado durante as práticas educativas. Este guia constitui-se em um modelo didático que faz uso do banco de dados públicos (NCBI) e softwares de Bioinformática com acesso livre. ABSTRACTBioinformatics can be understood as an interdisciplinary field of knowledge, whose goal is to use computational techniques, physics and mathematics to evaluate, create and manipulate biological information. Thus, this work aims to encourage the continued use of Bioinformatic softwares during ministration of long distance courses of Biochemistry by making use of a summarized guide presented here which may be employed during the practical activities. This guide constitutes an educational model based on the use of the public data bank (NCBI) and open-source Bioinformatic softwares.

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In recent years, the clinical and scientific interest in antibiotics known as polymyxin has increased greatly due to the large number of reports of multiresistant Gram-negative bacteria, among them P. aeruginosa. This work aimed to investigate proteins responsible for resistance to polymyxins encoded in P. aeruginosa genomes using in silico tools. To do so, E. coli MCR1 protein was used as bait. Sequences with similarities to MCR1 encoded in P. aeruginosa were determined. These proteins are between 465 and 521 amino acids in length. Molecular masses between 52.06-57.58 kDa, isoelectric point between 5.83 to 8.06, instability index between 60.33 to 66.42, aliphatic index between 99.980 to 107.39 and the hydropathy index between-0.038 to 0.037. These proteins belong to the DUF1705 superfamily with a Bit-score between 592,806 and 608,599. In conclusion the results evidenced the high degree of similarity between EpTAs including amino acids number, molecular mass, isoelectric point, instability index, aliphatic and hydrophobicity index, as well as secondary structures and protein domain with other proteins that confer resistance to polymyxins present in Gram-negative bacterial species of clinical interest. However, further studies are needed to identify the actual contribution of EptAs in P. aeruginosa species.