Saat ini di dunia sedang dilanda oleh sebuah penyakit yang disebabkan oleh virus SARS-CoV 2 atau yang dikenal dengan COVID-19. Pada orang lanjut usia dan memiliki riwayat penyakit kronis dapat mengakibatkan munculnya komplikasi yang lebih buruk. Seseorang dengan kadar glukosa darah tidak terkontrol, akan membuat prognosis COVID-19 semakin memburuk dan menyebabkan kematian. Puskesmas Trosobo, terdapat 50 anggota peserta prolanis yang aktif dengan kegiatan aktifnya setiap bulan. Saat pandemi Covid-19 kegiatan Prolanis terhambat. Kegiatan pengabdian masyarakat yang kami laksanakan bertujuan untuk mengetahui pentingnya peranan edukasi pola hidup sehat untuk mengkontrol kadar glukosa darah puasa anggota Prolanis di Puskesmas Trosobo selama pandemi Covid-19. Mayoritas anggota prolanis yang menjadi peserta kegiatan pengmas adalah berjenis kelamin perempuan, berusia 55-65 tahun, latar belakang pendidikan SMA, bekerja sebagai Ibu Rumah Tangga (IRT), dan tidak teratur dalam keteraturan kontrol berobat selama pandemi COVID-19. Pemberian edukasi pola hidup sehat pada masa pandemi COVID-19 terbukti efektif dan memberikan pengaruh yang signifikan mulai dari perubahan pola hidup sehat (p value = 0,043) dan penurunan kadar Glukosa Darah Puasa (p value = 0,043) pada anggota Prolanis Puskesmas Trosobo, Kabupaten Sidoarjo.

Nasopharyngeal swab collection for SARS-CoV-2 examination by RT-PCR requires Viral Transport Medium (VTM) using VTM non-activated and inactivated. This study determined the difference in CT values for SARS CoV-2 RT-PCR examination in non-activated VTM and inactivated VTM. This study is a comparative analytical study with a cross-sectional method using 20 confirmed positive samples for SARS-CoV-2 at RSU. Dr. Suyoto was analyzed by the T-Independent test (N gene) and Mann-Whitney test (ORF1ab gene) using SPSS with α=0.05. The results showed that VTM non-inactivated had a mean CT value of the N gene 33.56 ± 1.39 and the ORF1ab gene 35.4 ± 1.94, while VTM inactivated had an average CT value of 32.24 ± 1.44 for the N and ORF1ab gene 33.93 ± 3.22. Statistical analysis was tested by the T-independent test (N gene), and the Mann Whitney test (ORF1ab gene) between the use of VTM non-inactivated and VTM inactivated in the SARS CoV-2 RT-PCR examination, obtained p-value 0.05 with a mean difference the results of Ct gene n (p=0.006) and gene ORF1ab (p=0.028). This indicated a difference in the mean C CT value of the SARS CoV-2 RT-PCR examination in non-activated VTM and inactivated VTM.

Nasopharyngeal swab collection for SARS-CoV-2 examination by RT-PCR requires Viral Transport Medium (VTM) using VTM non-activated and inactivated. This study determined the difference in CT values for SARS CoV-2 RT-PCR examination in non-activated VTM and inactivated VTM. This study is a comparative analytical study with a cross-sectional method using 20 confirmed positive samples for SARS-CoV-2 at RSU. Dr. Suyoto was analyzed by the T-Independent test (N gene) and Mann-Whitney test (ORF1ab gene) using SPSS with α=0.05. The results showed that VTM non-inactivated had a mean CT value of the N gene 33.56 ± 1.39 and the ORF1ab gene 35.4 ± 1.94, while VTM inactivated had an average CT value of 32.24 ± 1.44 for the N and ORF1ab gene 33.93 ± 3.22. Statistical analysis was tested by the T-independent test (N gene), and the Mann Whitney test (ORF1ab gene) between the use of VTM non-inactivated and VTM inactivated in the SARS CoV-2 RT-PCR examination, obtained p-value 0.05 with a mean difference the results of Ct gene n (p=0.006) and gene ORF1ab (p=0.028). This indicated a difference in the mean C CT value of the SARS CoV-2 RT-PCR examination in non-activated VTM and inactivated VTM.

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