The objective of this work is to apply Atomic Force Microscopy in Peak Force mode to obtain topographic characteristics (mean roughness, root-mean-square roughness, skewness, kurtosis) and mechanical characteristics (adhesion, elastic modulus) of HighlightsThe Peak Force mode of AFM provides topographic and mechanical properties.The worn CLs show significant changes in respect to the un-worn CLs.The AFM results can be correlated with clinical test results.The elastic modulus is not the only parameter to consider regarding CL comfort.3