Two experiments were conducted to establish responses in milk Se concentrations in grazing dairy cows to different amounts of dietary Se yeast, and to determine the effects of the Se concentration of the basal diet. The hypothesis tested was that the response in milk, blood, and tissue Se concentrations to supplemental Se would not be affected by whether the Se was from the basal diet or from Se yeast. In addition, by conducting a similar experiment in either early (spring; experiment 1) or late (autumn; experiment 2) lactation, we hypothesized that different Se input-output relationships would result. Both 6-wk experiments involved 60 multiparous Holstein-Friesian cows, all of which had calved in spring. They were allocated to 1 of 10 dietary Se treatments that included 2 types of crushed triticale grain (low Se, approximately 165 microg of Se/kg of DM; or high Se, approximately 580 microg/kg of DM) fed at 4 kg of DM/d, and 1 kg of DM/d of pellets formulated to carry 5 quantities of Se yeast (0, 4, 8, 12, or 16 mg of Se). Daily total Se intakes ranged from <2 to >18 mg/cow in both experiments. Milk Se concentrations plateaued after 15 and 7 d of supplementation in experiments 1 and 2, respectively, and then remained at plateau concentrations. Average milk Se concentrations for the plateau period increased as the amount of Se yeast increased, and low- and high-Se grain treatments were different at all quantities of Se yeast, although there was a tendency for this difference to diminish at the greatest concentrations of yeast. There were significant positive, linear relationships between Se intake and the concentrations of Se in milk, which were not affected by the source of Se, and the relationships were similar for both experiments. Therefore, the output of Se in milk in experiment 1 was greater than that in experiment 2 because the milk yield of the cows in early lactation was greater. The estimated proportions of Se partitioned to destinations other than milk and feces increased with the amount of Se in the diet and were greater in experiment 2 than in experiment 1, a result that was supported by Se concentrations in whole blood and plasma and in semitendinosus muscle tissue. If high-Se products are to be produced for human nutrition, it is important to be able to develop feeding systems that produce milk with consistent and predictable Se concentrations so that products can consistently meet specifications. The results indicate that this objective is achievable.

Two experiments were conducted in central Vietnam to test the hypothesis that supplementation with a concentrate, comprising rice bran (45% fresh basis), maize (49%), fish meal (3%), urea (2%) and salt (1%), up to 2% of live weight (LW)/d (dry matter (DM) basis) would linearly increase digestible organic matter intake and LW gain of yellow cattle. In both experiments, there were five treatments, namely a basal diet of fresh grass fed at 1.25% of LW (experiment 1, elephant grass, Pennisetum purpureum; experiment 2, native grass) and rice straw (Oryza sativa) fed ad libitum or this diet supplemented with concentrate at about 0.3, 0.7, 1.3 or 2.0% LW. There were 4 male growing cattle per treatment in experiment 1 and 3 in experiment 2. Diets were fed for 44 (experiment 1) or 49 (experiment 2) days, with feed intake recorded daily, LW measured about weekly and digestibility measurements made over 7 days commencing on day 24 (experiment 1) or day 10 (experiment 2). The elephant grass and native grass had neutral detergent fibre (NDF) concentrations of 82 and 73% DM, and nitrogen concentrations of 1.3 and 1.8% DM, respectively. The rice straw used had a NDF concentration of 79-84% DM and nitrogen concentration of 0.8% DM. The concentrate had NDF and nitrogen concentrations of 33 and 2.8% DM. In both experiments, DM intake increased (p<0.001) linearly as the amount of concentrate consumed increased. Rice straw intake declined (p<0.001) (experiment 1: 1.24 to 0.48 kg DM/d; experiment 2: 0.95 to 0.50 kg DM/d) as concentrate intake increased. Grass intake was not significantly affected by concentrate intake in either experiment. The lowest amount of concentrate supplement increased forage intake, after which substitution rate increased as the amount of concentrate consumed increased. However, substitution rates at the highest amount of concentrate consumed were modest at 0.3 to 0.5 kg DM reduction in forage intake/kg DM supplement consumed. In both experiments, digestible organic matter intake increased linearly (p<0.001) (experiment 1: 1.16 to 2.38 kg/d; experiment 2: 1.30 to 2.49 kg/d) as the amount of supplement consumed increased, as did LW gain (experiment 1: 0.15 to 0.81 kg/d; experiment 2: 0.15 to 0.77 kg/d). This was associated with significant (p<0.01) linear increases in organic matter intake and apparent organic matter digestibility. Neutral detergent fibre digestibility declined as concentrate intake increased, but the effect was not significant (p = 0.051) in experiment 2. These results are discussed in relation to existing literature and potential to improve the profitability of cattle fattening in central Vietnam.

Sixteen cows in early lactation were individually fed diets consisting of fresh Persian clover (Trifolium resupinatum)-dominant pasture, offered to all cows at 3.7 kg DM/100 kg liveweight (LW); either alone or supplemented with amounts of crushed wheat ranging from ~0.3 to 0.9 kg DM/100 kg LW (four treatments with four cows per treatment). Cows fed Persian clover alone consumed 19 kg DM/day and total DM intake increased (P < 0.001) in a linear manner as the amount of wheat consumed increased, with no significant effects on clover intake. As the proportion of wheat in the diet increased, dietary neutral detergent fibre (NDF) concentrations declined from 28 to 24%, and in vivo NDF (P = 0.055) and acid detergent fibre (ADF; P = 0.015) digestibilities also declined. There were no significant effects of proportion of wheat in the diet on apparent digestibility of DM, organic matter or gross energy. The extent to which negative associative effects on NDF digestion was associated with the clover could not be determined as it was not possible to distinguish between the NDF derived from clover or wheat, but the decline in ADF digestibility suggested that most of the response lay with the clover since the wheat only contained relatively small amounts of ADF. Ruminal fluid pH was below 6.0 for more than 18 h/day in all cows. There was no effect of wheat in the diet on average ruminal fluid pH, but lowest values during the day were negatively related (P < 0.05) to the proportion of wheat in the diet. As the proportion of wheat in the diet increased, ruminal fluid ammonia-N concentration (P < 0.001) and the acetate + butyrate to propionate ratio (P < 0.001) decreased. The proportion of wheat in the diet did not affect nylon bag estimates of NDF degradation rates for grain or forage. Although most data indicated that effects of proportion of wheat in the diet on the utilisation of consumed nutrients were small, the marginal milk response to additional wheat averaged only 0.9 kg energy-corrected milk/kg DM wheat.

Sixteen cows in mid-lactation (milk yield of 23.8 +/- 2.3 kg/d) were individually fed diets consisting of chopped perennial ryegrass hay, offered at 3 kg of dry matter (DM)/100 kg of body weight (BW), fed either alone or supplemented with amounts of crushed wheat ranging from 0.4 to 1.6 kg of DM/100 kg of BW (increasing at nominal intervals of 0.4 kg of DM/100 kg of BW; 5 nominal treatments in total). Three cows were allocated to each treatment except the mid-range wheat treatment, which had 4 cows. Results were analyzed by regression because the intake of the wheat by cows within treatments varied. The hay was used to reflect the characteristics of summer pastures in southeastern Australia. Feed intake and fecal output were measured to determine digestion coefficients, feeds were incubated in nylon bags in the rumen, and rumen variables were monitored. Estimates of metabolizable energy (ME) of the hay from in vivo or in vitro digestibility were also compared. The digestibility of neutral detergent fiber (NDF) was depressed linearly as the amount of crushed wheat consumed increased to 36% of DM intake. The extent to which negative associative effects on NDF digestion were associated with the hay could not be determined, as it was not possible to distinguish between the NDF from hay and that from wheat. However, acid detergent fiber (ADF) digestion also declined, suggesting that most of the response lay with the hay because ADF was negligible in the wheat. Most data indicated that effects of proportion of wheat in the diet on the utilization of consumed nutrients were small. Despite substitution of wheat for hay reducing the forage intake of cows, there was a positive linear effect on marginal milk responses (1.3 kg of energy-corrected milk/kg of DM wheat). Mean rumen fluid pH declined as the proportion of wheat in the diet increased. The lowest pH for any individual cow during a 24-h period was 5.4, and the amount of time that rumen fluid pH was <6.0 ranged from 0 to 14 h depending on the amount of wheat consumed. It was concluded that these perturbations of the rumen environment were probably sufficient to result in negative associative effects. In addition, estimates of the ME content of the hay were higher when calculated from in vitro compared with in vivo digestibility, which has implications when estimating the amount of feed required for production.

Dairy production systems in south-eastern Australia are based primarily on grazed pasture. Perennial ryegrass (Lolium perenne L.) is the major grass species used in this region and farmers are faced with the challenge of choosing from more than 60 commercially available cultivars. This paper describes the development of a system termed as a forage value index that ranks the overall performance of perennial ryegrass cultivars relative to cultivar Victorian according to the summation of the estimated difference in the value of seasonal dry-matter (DM) yield of the cultivars. Average predicted seasonal DM yields were calculated by analysing the results of eight available perennial ryegrass plot trials across south-eastern Australia, using a multi-environment, multi-harvest linear mixed model. The differences in the model-predicted DM yield of each cultivar was compared with cultivar Victorian in each of five seasonal periods (autumn, winter, early spring, late spring, summer) to generate a series of performance values (1 per period) for each cultivar. Each performance value was then multiplied by an economic value (AU$/kg extra pasture grown) relating to each of four regions (Gippsland, northern Victoria, south-western Victoria, Tasmania) and seasonal period and aggregated to generate an overall forage value index rating for each cultivar. Economic values ranged from AU$0.11 to AU$0.39 per extra kilogram of DM grown, depending on the season and region, which translated into estimated benefits on dairy farms of up to AU$183 per ha per year for farmers that use high-yielding cultivars in place of cultivar Victorian perennial ryegrass.