Bacteriocin 2a is produced by a Lactobacillus sake strain isolated from Brazilian sausage. This bacteriocin was purified 72,000 fold by acid extraction from L. sake 2a, followed by cation exchange and reverse phase chromatography. Bacteriocin 2a dissipated the proton motive force in Listeria monocytogenes and reduced the intracellular ATP with no detectable increase in extracellular ATP. Bacteriocin 2a also mediated a concentration‐dependent efflux of carboxyfluorescein from liposomes made from L. monocytogenes lipids. These data indicate that bacteriocin 2a forms pores in the membrane of target cells.
Neste artigo, será discutida a contaminação do queijo Minas frescal por bactérias que podem causar toxinfecção alimentar, cujos sintomas podem ser diarréia, vômitos, febre, flatulência e até aborto. O queijo Minas frescal, assim como outros alimentos, é fabricado com leite cru, que favorece o crescimento e a presença de um elevado número de bactérias patogênicas como Staphylococcus aureus, Salmonella spp., Shigella spp., Escherichia spp. e Listeria spp. O controle de contaminação microbiana e as boas práticas de fabricação desse queijo são de grande importância para a saúde pública, pois esse produto é muito consumido pela população.
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