In this study we evaluate the influence of cadmium (Cd) on the spores from Athyrium filix-femina (Linnaeus) Roth (1799), Dryopteris filix-mas (Linnaeus) Schott ( 1834) and D. affinis (Lowe) Fraser-Jenkins (1979) starting with the gametophyte and sporophyte differentiation and continued with the biochemical changes. For spores cultivation we used soil treated with Knop solution in which different amounts of Cd acetate were dissolved and we obtained the folowing variants: V1Cd = 0,01g Cd 2+ kg -1 ; V2Cd = 0,02 g Cd 2+ kg -1 ; V3Cd = 0,05 g Cd 2+ kg -1 ; V4Cd = 0,1 g Cd 2+ kg -1 . For 4 months we monitored the gametophyte and sporophyte differentiation and after that we determinate the amount of photosynthetic pigments and the content of polyphenols. Over time, the differences between the variants regarding the differentiation of gametophyte were reduced, and the sporophyte appeared in all variants. The smallest concentration of Cd stimulate the amount of assimilatory pigments and the content of polyphenols increased at the highest concentrations of Cd.
Plants based synthesis of Ag and Au nanoparticles represent one of the most important focus of researchers due to their applications and catalytic activities. We utilized various techniques to obtain extracts with bimetallic nanoparticles from Asplenium scolopendrium leaves; we tested the following variants: M -microwave extract, M NP -microwave extract with nanoparticles Au-Ag, C -extract obtained in the oven, C NP -extract obtained in the oven with nanoparticles Au-Ag, H -hydroalcoholic solution; for each extract were made 2 dilutions: 1:10 and 1:100. The germinative percent was used to quantify the effect of the extracts with/without bimetallic nanoparticles on Asplenium scolopendrium and Dryopteris filix-mas spores. In the variants with bimetallic nanoparticles, regardless species, dilution or obtaining technique, after 1 week no germination was recorded and after one month the situation was the same. Also, no germination was observed in the variants without bimetallic nanoparticles with 1:10 dilution. In both species, at 1:100 dilution, the variants with the extract obtained using microwave technique (without nanoparticles) recorded a higher germinative percent than the other variants with extract.
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