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Root phenotyping describes methods for measuring root properties, or traits. While root phenotyping can be challenging, it is advancing quickly. In order for the field to move forward, it is essential to understand the current state and challenges of root phenotyping, as well as the pressing needs of the root biology community. In this letter, we present and discuss the results of a survey that was created and disseminated by members of the Graduate Student and Postdoc Ambassador Program at the 11th symposium of the International Society of Root Research. This survey aimed to (1) provide an overview of the objectives, biological models and methodological approaches used in root phenotyping studies, and (2) identify the main limitations currently faced by plant scientists with regard to root phenotyping. Our survey highlighted that (1) monocotyledonous crops dominate the root phenotyping landscape, (2) root phenotyping is mainly used to quantify morphological and architectural root traits, (3) 2D root scanning/imaging is the most widely used root phenotyping technique, (4) time-consuming tasks are an important barrier to root phenotyping, (5) there is a need for standardised, high-throughput methods to sample and phenotype roots, particularly under field conditions, and to improve our understanding of trait-function relationships.

Soil salinisation and overgrazing are two important factors limiting plant growth in the Songnen Grassland, Northeast China. Leymus chinensis, a dominant rhizomatous grass, resists grazing and tolerates saline–alkali stress. However, its adaptive mechanisms to the dual effects of grazing and saline–alkali stress remain largely unknown. A two-factorial field experiment was conducted in two consecutive years in the natural L. chinensis community, combining the addition of mixed saline–alkali solution (NaCl:NaHCO3:Na2CO3 1:1:1, amount 559.13 g m–2 year–1) with clipping (removal of 60% of aboveground biomass, AGB). Saline–alkali addition significantly increased AGB and total biomass in the no clipping but not in the clipping treatment. Irrespective of clipping, ramet density was significantly decreased, and individual ramet biomass was significantly increased under salt stress. The significant increase in AGB was due to a high K+:Na+ ratio, high water-use efficiency, and an increase in leaf area index and net photosynthesis rate of individual ramets under salt–alkali stress. Clipping significantly decreased AGB and total biomass regardless of saline–alkali addition, possibly because of decreased sugar content of rhizomes. Saline–alkali and clipping had an interactive effect on AGB and total biomass of L. chinensis. The significant reduction in AGB and total biomass were mainly caused by reduced proline and water-soluble carbohydrate content under dual stress. A modified and simplified graphic model of the limiting resource model was proposed based on our results. Leymus chinensis can grow well under saline–alkali stress via ramet biomass compensation, in which the significant decrease in ramet density is compensated by the significant increase in individual ramet biomass. Ramet compensation and clonal integration were identified to be main mechanisms of herbivory and saline–alkali tolerance.