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A novel diamine monomer 1, 4,4'-(9H-fluorene-9,9-diyl)-bis(2-tert-butylaniline), was synthesized from 9-fluorenone and 2-tert-butylaniline by the condensation reaction. Then it was polymerized with several commercial aromatic dianhydrides, respectively, to produce polyimides (PIs) by the one-pot method. The number-averaged molecular weights of the resulting PIs are in the range of (4.54-8.82) 3 10 4 with polydispersity indices from 2.51 to 4.33 by gel permeation chromatography measurement. They are soluble in many organic solvents and can form transparent and tough films by solution-casting. The cut-off wavelengths of UV-vis absorption for the PI films are below 360 nm, which are much lower than that of Kapton film. The light transparency of them is above 90% in the visible light range from 400 to 760 nm. They also display relatively low dielectric constants (from 2.79 to 3.00), low water absorption rates (<1%), and high tensile strength (> 50 MPa). Their excellent solubility and transparency can be attributed to the incorporation of tert-butyl groups and fluorene units into the rigid backbones of PIs. Simultaneously, they still maintain the high thermal stability (the 5% weight loss temperature in the range from 526 to 539 8C in nitrogen) and the high glass transition temperatures (T g > 340 8C).

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Skeletal muscle development plays an important role in muscle quality and yield, which decides the economic value of livestock. Long non-coding RNAs (lncRNAs) have been reported to be associated with skeletal muscle development. However, little is revealed about the function of lncRNAs in rabbits’ muscle development. LncRNAs and mRNAs in two rabbit breeds (ZIKA rabbits (ZKR) and Qixin rabbits (QXR)) with different growth rates at three developmental stages (0 day, 35 days, and 84 days after birth) were researched by transcriptome sequencing. Differentially expressed lncRNAs and mRNAs were identified for two rabbit breeds at the same stages by DESeq package. Co-expression correlation analysis of differentially expressed lncRNAs and mRNAs were performed to construct lncRNA–mRNA pairs. To explore the function of lncRNAs, Gene Ontology (GO) analysis of co-expression mRNAs in lncRNA–mRNA pairs were performed. In three comparisons, there were 128, 109, and 115 differentially expressed lncRNAs, respectively. LncRNAs TCONS_00013557 and XR_518424.2 differentially expressed in the two rabbit breeds might play important roles in skeletal muscle development, for their co-expressed mRNAs were significantly enriched in skeletal muscle development related GO terms. This study provides potentially functional lncRNAs in skeletal muscle development of two rabbit breeds and might be beneficial to the production of rabbits.

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