Recent advances in image technology, including significant gains in spatial resolution, have made realtime sequential ovarian evaluations possible in small rodents, allowing longitudinal (continued) studies of the ovarian cycle and reducing the required number of experimental animals. The aim of this study was to evaluate exogenous stimulated follicular growth in mice using high-resolution ultrasound technology. Female mice (n ¼ 15) received a 5 IU intraperitoneal injection of equine chorionic gonadotropin (eCG) and 48 h later a 5 IU injection of human chorionic gonadotropin (hCG), and were allowed to mate thereafter. In experiment 1, animals (n ¼ 7) were evaluated every 6 h, from 3 to 51 h after eCG injection, with an ultrasound biomicroscopy (UBM) equipped with a realtime 45 MHz microvisualization probe (RMV 707b). The ovaries were identified and follicular population quantified, and follicles were classified according to the diameter as small ( 449 mm) or large (!450 mm). A significant change in the distribution of follicle population according to category was observed only 45 h after eCG injection (P , 0.05). In experiment 2, animals (n ¼ 8) were evaluated every 2 h, from 2 h to 10 h after hCG treatment. The largest follicles reached a maximum size (596.7 + 106.0 mm) 5.8 + 2.3 h after hCG injection. As expected, the population of large follicles decreased thereafter, indicating the progress of ovulations, but large follicles were still detected late after treatment (10.1 + 1.1 h). In conclusion, UBM can be used to evaluate follicle dynamics in superstimulated mice (C57BL/6 and BALB/c); significant changes in follicle distribution only occur at later stages after eCG stimulation; and hCG-induced ovulations may not occur synchronously in mice.
A special mold with mechanically driven mold surface motions (rotation and axial) was developed allowing the imposition of different molding sequences, thus enlarging the possibilities of controlling the thermomechanical environment, and consequently the manipulation of the developed morphology. In this work, centre gated discs were injection molded with distinct rotation velocities of the mold surface. The moldings were characterized by several structure sensitive techniques. The imposition of a mold surface rotation has a marked effect on the morphology of the moldings, with novel molding morphologies being developed: a laminated skin–core structure featuring a dual shish‐kebab structure differently oriented relatively to the flow direction, one in each of the skin layer.magnified image
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