RESUMENEl objetivo del presente estudio fue valorar la prueba del azul brillante de cresilo (BCB) como método indirecto para seleccionar ovocitos competentes para la producción in vitro de embriones (PIV). Los complejos cúmulos-ovocitos (COC) fueron obtenidos de dos vaquillas criollas sometidas a dos tratamientos: T1 = COC recuperados por OPU (ovum pick-up) previa estimulación con FSH-LH; T2 = COC recuperados sin previa estimulación de la donante (testigo). Las dos vaquillas fueron alternadas en los dos tratamientos y se hicieron cinco repeticiones. Los COC recuperados fueron clasificados en tipos A, B, C y D. Luego se aplicó la prueba del BCB a cada uno de los tipos de COC para determinar si son BCB+ o BCB-. T1 permitió recuperar 5.2 más COC que T2 (p<0.05). Al aplicar la prueba del BCB se determinó que todos los ovocitos tipo A de T1 y T2 fueron BCB+; es decir, terminaron su crecimiento y se encontraban listos para iniciar el proceso de maduración in vitro; sin embargo, alrededor del 50% de los COC tipo B, C y D de T1 y T2 fueron BCB+. Se concluye que la selección de COC basado en las características morfológica es un método confiable únicamente para los de tipo A, y tiene un 50% de error para los COC de tipo B, C y D y, por lo tanto, la aplicación de la prueba del BCB permite mejorar esta selección de forma no invasiva.
The influence of additives on the structural behaviour of cement-stabilised rammed earth was investigated through an experimental programme based on 13 different mixes with varying cement contents (6, 8 and 10% by earth weight) and three different types of additives (superplasticiser, air-entraining agent (AEA) and hardening accelerator). In total, 156 specimens were fabricated and tested at 7 and 28 days in order to compare their compressive strength and other properties such as stress–strain relationships, shrinkage and workability. The results highlighted the essential role of cement in increasing the compressive strength of the material. Specifically, cement contents of 6%, 8% and 10% corresponded, respectively, to strength increases of 41%, 80% and 92% compared with the reference mix with a mean compressive strength of 1.15 MPa. The AEA was found to be the most effective additive for increasing the compressive strength, with obtained strengths of up to 2.93 MPa (an increase of 155% compared with the reference mix). The efficiency of each mix was evaluated through a strength/cost ratio. In addition, an overall comparison of compressive strength, shrinkage, deformation, cost and workability was performed using an analytic hierarchy process. Finally, the experimental results were compared with the mechanical requirements of various codes.
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