Six secondary metabolites, including two novel iridoids, longifolides A (1) and B (2), were isolated by various chromatographic methods from a methanol extract of branches and leaves of Morinda longifolia Craib. The structures of the compounds were determined on the basis of NMR spectroscopic (1 H and 13 C NMR, HSQC, HMBC, 1 H-1 H COSY, NOESY) and FTICR-MS data, as well as by comparison of them with literature values.

From the methanolic extract of the fruits of Gleditschia australis Hemsl., a new flavonoid derivative 3′′-Omenthiafoloylisovitexin (1) and a new carbohydrate ester of cinnamic acid 1-O-E-cinnamoyl-[2-O-E-cinnamoyl-α-Lrhamnopyranosyl-(1→6)-β-D-glucopyranoside] (2) have been isolated along with four known compounds, 1-O-E-cinnamoyl-[3-O-E-cinnamoyl-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside], isovitexin, luteolin, and quercetin. Their structures were elucidated on the basis of physical and spectroscopic evidence.

High performance liquid chromatography diode array detector and Ion trap MS combined with some statistical methods was developed for the quality control of Mallotus apelta with malloapelta B as marker compound. In the ESI-MS experiment, both negative and positive ESI modes were used for the detection of malloapelta B. All the results showed that the developed fingerprint assay was specific and could further serve for quality identification and quantitative the content of malloapelta B in the Mallotus sample.