Advances in single-molecule localization microscopy are providing unprecedented insights into the nanometer-scale organization of protein assemblies in cells and thus a powerful means for interrogating biological function. However, localization imaging alone does not contain information on protein conformation and orientation, which constitute additional key signatures of protein function. Here, we present a new microscopy method which combines for the first time Stochastic Optical Reconstruction Microscopy (STORM) super-resolution imaging with single molecule orientation and wobbling measurements using a four polarization-resolved image splitting scheme. This new method, called 4polar-STORM, allows us to determine both single molecule localization and orientation in 2D and to infer their 3D orientation, and is compatible with high labelling densities and thus ideally placed for the determination of the organization of dense protein assemblies in cells. We demonstrate the potential of this new method by studying the nanometer-scale organization of dense actin filament assemblies driving cell adhesion and motility, and reveal bimodal distributions of actin filament orientations in the lamellipodium, which were previously only observed in electron microscopy studies. 4polar-STORM is fully compatible with 3D localization schemes and amenable to live-cell observations, and thus promises to provide new functional readouts by enabling nanometer-scale studies of orientational dynamics in a cellular context.

The focus of this paper is a manufacturing system placed in Calabria (Italy) which produces high pressure hydraulic hoses. The objective is to implement a simulator to be used both for carrying out specific analysis devoted to increase system efficiency and for supporting the manufacturing process management (implementation of all the features required for using real time the simulator).After the modeling phase, the simulation model has been validated comparing simulation results with real system performances. In this paper the authors test tool potentials investigating system behavior under different scenarios obtained varying manufacturing departments configuration. The utilization degree of each manufacturing department and its daily production measure system performance.

Transmit/Receive Modules for Phased Array Radar are often identified as a key cost driver for the system. The cost structure of the module is driven by both the performance specifications and the choices made in design and manufacturing of the module. Seeking a path to dramtically lower the cost of T/R modules for phased array systems, commercial processes and practices have been adopted for the MMIC design, MMIC packaging and module construction. These new manufacturing approaches offer a path to cost reduction while maintaining a high level of performance.

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