The research aimed to evaluate the film membrane of Nano Chitosan Resveratrol (NCHR) for biological, physicochemical, and antibacterial properties. Psychochemically, the functional groups of chitosan compounds were examined by FTIR, chemical compounds by GCMS, and the morphology of chitosan and chemical elements by SEM-EDS. Biologically, the characteristics of NCHR were examined by solubility, swelling, permeability, and biodegradation tests. Meanwhile, the antibacterial properties were examined for inhibition of Porphyromonas gingivalis (P. gingivalis) ATCC 33277 by Minimal inhibition concentration (MIC) and growth assessment by spectrophotometry. Nano Chitosan (NCH) has appeared at 1033.85 cm-1 as a sharp peak indicating the P=O group and contains anti-toxicity compounds (Ethane, 1,1-diethoxy- (CAS) 1,1-Diethoxye) is 81.06% and antioxidant compounds Limonene is (1.28%). In addition, NCH has chemical elements, Oxygen Weight (69.4%), calcium (19.7%), magnesium (6.6%), and phosphorus (4.3%). NaCl 0.9%, PBS, and Aquades. In addition, it has an excellent index of water vapor transmission rate (WVTR) in all solvents (R2³ 0.95). The NCHR membrane film is bacteriostatic (≤ 300 CFU/mL) with each value of Minimal Inhibition Concentration (MIC) >15 mm. The Nano chitosan contains antitoxic, antioxidant, and antibacterial compounds with high oxygen elements. The film membrane of nano chitosan resveratrol can maintain the stability of changes in pH with a very high solubility index, swelling index, and WVTR index, as well as good biodegradation and antibacterial properties. Doi: 10.28991/ESJ-2023-07-03-012 Full Text: PDF

Context: Candida albicans is a pathological agent that triggers oral candidiasis because C. albicans can adapt to changes to increase growth and adhesion through biofilm formation mechanisms. Moringa oleifera Lam. has been reported to have fungistatic properties and increases the metabolism change of C. albicans. Aims: To evaluate the fungistatic effect of M. oleifera leaves ethanolic extract (MOLE) on the metabolism changes of C. albicans cells associated with growth and biofilm formation. Methods: The assessment of metabolism changes (stress response and metabolic alterations) of C. albicans by the action of MOLE was performed by mean of FTIR, growth assessment by spectrophotometry, biofilm formation with 1% crystal violet, also read by spectrophotometry, and observation of biofilm mass with a microscope. Results: MOLE showed substantial absorption values based on topological polar surface area (<140 Å2). Concentrations of 25% and 6.25% of MOLE increased the stress response (metabolism changes) of C. albicans (66-75%), meanwhile 50% and nystatin (100.000 IU/mL) were similar in inducing metabolism changes of C. albicans. All concentrations of M. oleifera could inhibit the growth of C. albicans at all incubation times (24, 48, and 72 h) with an Optical Density (OD) of 0.02-0.05 (<300 CFU/mL) and were able to degrade the biofilm formation of C. albicans on a scale substantial at 24 and 48 h (OD>0.4), and moderate scale at 72 h (OD 0.2-0.39). Conclusions: The extract of M. oleifera has increased metabolism changes (stress response) of C. albicans cells, which correlate with the ability to inhibit growth and biofilm formation for 24, 48, and 72 h.