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Dentists have been supposed to be among the healthcare workers at greatest risk of SARS-CoV-2 infection. However, scant data are available on the issue. The aim of this study is to quantify the SARS-CoV-2 antibody prevalence and determinants in a sample of dentists, dental hygienists, and other personnel employed among the dental staff in Lombardy region. We used an accurate rapid diagnostic test kit detecting immunoglobulins (Ig) in 504 adults. Of the 499 participants who obtained a valid antibody test, 54 (10.8%) had a SARS-CoV-2 positive test (0.4% IgM+, 1.8% both IgM+ and IgG+, and 8.6% IgG+). A statistically significant association with infection was found for geographic area (compared to Milan, adjusted odds ratio was 2.79, 95% confidence interval, CI: 1.01–7.68 for eastern and 2.82, 95% CI: 1.34–5.94, for southern Lombardy). The clinical staff did not result positive to SARS-CoV-2 more frequently than the administrative staff. This is the first study using antibody test in the dental staff personnel. It shows that the prevalence of SARS-CoV-2 infection in Lombardy region was around 10%, in line with estimates on other healthcare professionals. Despite the close physical contact with the patient, dentists have been able to scrupulously manage and effectively use protective devices.

Aim. The aim of this work is to describe a case of immediate implant placement after extraction of the upper right first premolar, with the use of CAD/CAM technology, which allows an early digital impression of the implant site with an intraoral scanner (MHT 3D Progress, Verona, Italy). Case Report. A 46-year-old female was referred with a disorder caused by continuous debonding of the prosthetic crown on the upper right first premolar. Clinically, there were no signs, and the evaluation of the periapical radiograph showed a fracture of the root, with a mesial well-defined lesion of the hard tissue of the upper right first premolar, as the radiolucent area affected the root surface of the tooth. It was decided, in accordance with the patient, that the tooth would be extracted and the implant (Primer, Edierre implant system, Genoa, Italy) with diameter of 4.2 mm and length of 13 mm would be inserted. After the insertion of the implant, it was screwed to the scan abutment, and a scan was taken using an intraoral scanner (MHT 3D Progress, Verona, Italy). The scanned images were processed with CAD/CAM software (Exocad DentalCAD, Darmstadt, Germany) and the temporary crown was digitally drawn (Dental Knowledge, Milan, Italy) and then sent to the milling machine for production with a composite monoblock. After 4 months, when the implant was osteointegrated, it was not necessary to take another dental impression, and the definitive crown could be screwed in. Conclusion. The CAD/CAM technology is especially helpful in postextraction implant for aesthetic rehabilitation, as it is possible to immediately fix a provisional crown with an anatomic shape that allows an optimal healing process of the tissues. Moreover, the removal of healing abutments, and the use of impression copings, impression materials, and dental stone became unnecessary, enabling the reduction of the chair time, component cost, and patient's discomfort. However, it is still necessary for scientific research to continue to carry out studies on this procedure, in order to improve the accuracy, the reliability, and the reproducibility of the results.

This review aims at providing an update of the current European Union (EU) legislation on feed-related issues. Regulations and Directives were classified into the following categories: general food law, placing on the market and use of feed, official controls, sampling and analysis, hygiene, undesirable substances, additives, animal by-products, OGMs, feed intended for particular nutritional purposes, and organic production. An overview of all laws related to the above-mentioned topics is given, and the main points of each law, cited in conjunction with its effect on previous laws (replacement, modification, amendments, and main related acts), are reported in tables.

The need for global feed supply traceability, the high-throughput testing demands of feed industry, and regulatory enforcement drive the need for feed analysis and make extremely complex the issue of the control and evaluation of feed quality, safety, and functional properties, all of which contribute to the very high number of analyses that must be performed. Feed analysis, with respect to animal nutritional requirements, health, reproduction, and production, should be multianalytically approached. In addition to standard methods of chemical analysis, new methods for evaluation of feed composition and functional properties, authenticity, and safety have been developed. Requirements for new analytical methods emphasize performance, sensitivity, reliability, speed, simplified use, low cost for high volume, and routine assays. This review provides an overview of the most used and promising methods for feed analysis. The review is intentionally focused on the following techniques: classical chemical analysis; in situ and in vitro methods; analytical techniques coupled with chemometric tools (NIR and sensors); and cell-based bioassays. This review describes both the potential and limitations of each technique and discusses the challenges that need to be overcome to obtain validated and standardized methods of analysis for a complete and global feed evaluation and characterization.