The time-course of disintegration of neurons, and the concomitant reactions of various types of glial cells, in the dorsal nucleus of the lateral geniculate body (LG) were studied in 12 Rhesus monkeys sacrificed in groups of two at time intervals of .l, 3, 6, 12, 25 and 50 weeks following unilateral removal of the occipital lobe. The change in the mean number of neurons, astrocytes, oligodendrocytes and microglia, found per unit of volume, in Nissl preparations, of the degenerated LG, was expressed as a percentage of the mean number of the corresponding cells estimated in the normal LG on the same slide. The number of degenerating neurons decreased progressively. While 12 weeks following surgery a few isolated nerve cell nuclei could still be seen in the LG, by the end of the twenty-fidth postoperative week the nucleus was practically devoid of neurons. The reaction of the astrocytes indicated a biphasic processproliferation with subsequent reversal to an almost normal level. The oligodendrocytes and microglia multiplied at a somewhat slower rate, but remained far above the control values over a period of 50 postoperative weeks.

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Acute monophasic experimental autoimmune encephalomyelitis (AMEAE) was induced in 5 Macacus cynomolgus monkeys. Serum and cerebrospinal fluid (CSF) paired samples were collected before and four weeks after immunization, time of complete development of AMEAE clinical picture. After immunization the CSF/serum albumin ratio and the IgG index were markedly increased. Agarose isoelectric focusing (AIEF) followed by immunofixation revealed faint IgG oligoclonal bands in both serum and CSF of all monkeys, which became more evident after Kappa and Lambda assessment. Intrathecal synthesis of lgG oligoclonal bands was detected in only one CSFAffinity-driven immunoblotting failed to detect anti-myelin basic protein (MBP) oligoclonal IgG. No free light chain (FLC)patterns or IgA and IgM oligoclonal bands were detected.

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Novos métodos simples e confiáveis para a determinação de ácido úrico (UA) são propostos e validados. Para a determinação quantitativa de UA, duas matrizes foram usadas: a reação oscilatória de Bray-Liebhafsky (BL) em um estado estacionário de não equilíbrio, estável, próximo ao ponto de bifurcação (método A), e o subsistema não oscilante (mistura de KIO 3 e H 2 SO 4), isto é, reação de Dushman (RD) em um estado estacionário (método B). Os métodos propostos são otimizados num reator tanque continuamente agitado (CSTR) e aplicados com excelentes resultados na determinação de UA em amostras de urina humana. A relação linear entre o deslocamento potencial máximo DE m e o logaritmo da concentração de UA (processo A), ou entre DE m e a concentração UA (processo B) é obtido no intervalo de concentração 2,98 × 10-5-2,68 × 10-4 mol L-1 e 2,98 × 10-5-3,58 × 10-4 mol L-1 , respectivamente. Os métodos têm uma velocidade de processamento de amostra excelente de 30 amostras h-1 (método A) e 7 amostras h-1 (método B) com sensibilidade determinada para ser 1,1 × 10-5 mol L-1 (método A) e 8,9 × 10-6 mol L-1 (método B), e precisão RSD ≤ 3.4% para ambos os métodos. Alguns aspectos do possível mecanismo de ação de UA nos sistemas de reação oscilante BL e não-oscilante de Duschman, são discutidos em detalhe. Simple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO 3 and H 2 SO 4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift DE m , and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 × 10-5-2.68 × 10-4 mol L-1 and 2.98 × 10-5-3.58 × 10-4 mol L-1 , respectively. The methods have an excellent sample throughput of 30 samples h-1 (method A) and 7 samples h-1 (method B) with the sensitivity determined to be 1.1 × 10-5 mol L-1 (method A) and 8.9 × 10-6 mol L-1 (method B) as well as the precision RSD ≤ 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail.