By the example of some phenols and qninones, in aqueous solutions possibilities for the quantitative determination are tested separately and in the mixture. Methods for thin‐layer chromatography and spectrophotometry were developed. There can be chromatographically deteeted between 5 and 80 μg of substance. Phenol and quinone concentrations of 1.5 · 10−3 … 0.5 · 10−4 M were spectrophotometrically investigated.
In two test series, for which 10 and 2 … 4 g/1 wet matter of higher and lower aquatic plants from the lake Baikal and from the waters in the surroundings of Irkutsk were used, phenol solutions having concentrations of 10−3 … 10−5 M (1 … 5 mg/1) were applied. During the investigations the possible error clue to the presence of microorganisms was eliminated. The phenol decomposition by aquatic plants proceeds as a first‐order reaction. The results of analyses are summarized in several tables and graphical representations.
Under laboratory conditions and conditions similar to practice in the shore region of the lake Baikal, Nitella was added to aqueous solutions of benzidine and α‐naphthylamine in the concentration range of 10−3 … 10−4 M. The results are summarized graphically in in tables. The two substances were accumulated in the protoplasm of the Nitella cells in considerable quantities.
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