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A new second order non-linear optical organic crystal Urea L-threonine has been grown by aqueous solution slow cooling technique. The grown crystal is non hygroscopic and transparent in the visible region. The solubility of the grown crystal was found. The crystal structure and perfection were determined using powder XRD and High resolution XRD. The molecular structure was confirmed by 1 H-NMR spectrum. Thermal properties of the crystal have been studied using thermogravimetric (TG) and differential thermal analysis (DTA). The dielectric response of the crystal with varying frequencies at different temperatures was studied. UV-Visible spectrum was recorded to study the optical transparency of the grown crystals. The second order non linear optical property was examined by Kurtz powder technique and found that the second order susceptibilities is 1.2 KDP at wavelength 1064 nm. The mechanical behaviour was studied by Vickers Microhardness test. The results are discussed in detail.