A digestibility experiment and subsequent 84-day feeding experiment evaluated the use of pea and canola meal products in diets for rainbow trout. The effect of milling and heat treatment on nutrient, dry matter and energy digestibility of raw/whole peas, raw/dehulled peas, extruded/dehulled peas and autoclaved air-classified pea protein was determined. Digestibility of the protein component was uniformly high for all pea ingredients (90.9-94.6%), regardless of the processing treatment. Autoclaving or extrusion increased starch digestibility by 41-75% (P £ 0.05), which consequently increased energy and dry matter digestibility of whole and dehulled peas. Autoclaved air-classified pea protein had superior protein (94.6%), energy (87.0%) and dry matter (84.0%) digestibility (P £ 0.05). It was demonstrated that inclusion of 25% dehulled peas, 20% airclassified pea protein or 20% canola meal fines was feasible in trout diets allowing for replacement of soya bean meal. The data showed no difference (P ‡ 0.05) in feed intake, final weight and specific growth rate (SGR) measurements, and feed utilization was not compromised with inclusion of pea or canola meal products as the primary plant ingredient. It was concluded that dehulled peas, air-classified pea protein and canola meal fines are suitable ingredients for use in trout diet formulation at a level of 20%.
KEY WORDS
A dephytinized protein concentrate prepared from canola seed (CPC) was assessed for nutrient digestibility and performance in rainbow trout (Oncorhynchus mykiss). The apparent digestibility coefficients of CPC were: dry matter, 817 g kg−1; crude protein, 899 g kg−1; gross energy, 861 g kg−1; arginine, 945 g kg−1; lysine, 935 g kg−1; methionine, 954 g kg−1; threonine, 893 g kg−1. A 9‐week performance trial assessed 7 diets. Fishmeal provided 940 g kg−1 of the protein in the control diet. Test diets consisted of CPC or water‐washed CPC replacing 500 and 750 g kg−1 of fishmeal protein; and CPC plus an attractant replacing 500 and 750 g kg−1 of fishmeal protein. No significant differences in performance were observed (P > 0.05). A subsequent 9‐week performance trial evaluated the effect of adding CPC into compound diets containing fishmeal/soybean meal/corn gluten meal. Five diets were prepared: fishmeal provided 670 g kg−1 of the protein in the control diet, in the remaining diets CPC was incorporated into commercial‐like trout diets at 100, 200 and 300 g kg−1 replacement of fishmeal protein, the fifth diet included an attractant in the 300 g kg−1 replacement diet. No significant differences in performance were obtained (P > 0.05). These studies show that dephytinized canola protein concentrate has potential to replace substantial levels of fishmeal in diets for carnivorous fish without compromising performance.
Two short‐term palatability experiments indicated that supplementation of diets containing 15.0% of canola meal or 30.5% of air‐classified pea protein with 3.3% or 3.9% thin distillers' solubles (dry matter basis), respectively, increased feed intake (P < 0.05) of rainbow trout over a 4‐day period. Supplementation of the same diets with 6.6% or 1.9% thin distillers' solubles, respectively, did not increase feed intake (P > 0.05). Supplementation of the diets with 1.0% of a commercial palatability enhancer did not increase feed intake (P > 0.05). A subsequent 12‐week growth experiment evaluated the long‐term feed intake enhancing capability of thin distillers' solubles. Supplementation of diets containing 20.0% of air‐classified pea protein or 20.0% of canola meal fines with thin distillers' solubles (4.0%, dry matter basis) over a 12‐week period had no effect on feed intake or growth of rainbow trout fed either diet (P > 0.05). The reduction in the palatability enhancing ability of thin distillers' solubles may be the result of the fish becoming accustomed to the taste of this product over a longer feeding period, or because of the possible destruction or alteration of the amino acids involved in gustatory stimulation during thermal processing.
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